Abstract
We report here a rapid evaporation method that produces in high yield giant unilamellar vesicles up to 50 microns in diameter. The vesicles are obtained after only 2 min and can be prepared from different phospholipids, including L-alpha-phosphatidylcholine (lecithin), dipalmitoleoyl L-alpha-phosphatidylcholine, and beta-arachidonoyl gamma-palmitoyl L-alpha-phosphatidylcholine. Vesicles can be produced in distilled water and in Hepes, phosphate, and borate buffers in the pH range of 7.0 to 11.5 with ionic strengths up to 50 mM. The short preparation time allows encapsulation of labile molecular targets or enzymes with high catalytic activities. Cell-sized proteoliposomes have been prepared in which gamma-glutamyltransferase (EC 2.3.2.2) was functionally incorporated into the membrane wall.
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- Dimitriadis G. J. Translation of rabbit globin mRNA introduced by liposomes into mouse lymphocytes. Nature. 1978 Aug 31;274(5674):923–924. doi: 10.1038/274923a0. [DOI] [PubMed] [Google Scholar]
- HANES C. S., HIRD F. J. R., ISHERWOOD F. A. Enzymic transpeptidation reactions involving gamma-glutamyl peptides and alpha-amino-acyl peptides. Biochem J. 1952 Apr;51(1):25–35. doi: 10.1042/bj0510025. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kremer J. M., Esker M. W., Pathmamanoharan C., Wiersema P. H. Vesicles of variable diameter prepared by a modified injection method. Biochemistry. 1977 Aug 23;16(17):3932–3935. doi: 10.1021/bi00636a033. [DOI] [PubMed] [Google Scholar]
- Lasic D. D. The mechanism of vesicle formation. Biochem J. 1988 Nov 15;256(1):1–11. doi: 10.1042/bj2560001. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meister A., Tate S. S. Glutathione and related gamma-glutamyl compounds: biosynthesis and utilization. Annu Rev Biochem. 1976;45:559–604. doi: 10.1146/annurev.bi.45.070176.003015. [DOI] [PubMed] [Google Scholar]
- Mueller P., Chien T. F., Rudy B. Formation and properties of cell-size lipid bilayer vesicles. Biophys J. 1983 Dec;44(3):375–381. doi: 10.1016/S0006-3495(83)84311-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Orwar O., Fishman H. A., Ziv N. E., Scheller R. H., Zare R. N. Use of 2,3-naphthalenedicarboxaldehyde derivatization for single-cell analysis of glutathione by capillary electrophoresis and histochemical localization by fluorescence microscopy. Anal Chem. 1995 Dec 1;67(23):4261–4268. doi: 10.1021/ac00119a010. [DOI] [PubMed] [Google Scholar]
- Ostro M. J., Cullis P. R. Use of liposomes as injectable-drug delivery systems. Am J Hosp Pharm. 1989 Aug;46(8):1576–1587. [PubMed] [Google Scholar]
- Sikka S. C., Kalra V. K. Gamma-glutamyl transpeptidase-mediated transport of amino acid in lecithin vesicles. J Biol Chem. 1980 May 25;255(10):4399–4402. [PubMed] [Google Scholar]
- Szoka F., Jr, Papahadjopoulos D. Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse-phase evaporation. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4194–4198. doi: 10.1073/pnas.75.9.4194. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Timofeev B. A., Bolotin I. M., Stepanova L. P., Bogdanov A. A., Jr, Georgiu K., Malyshev S. N., Petrovsky V. V., Klibanov A. L., Torchilin V. P. Liposomal diamidine (imidocarb): preparation and animal studies. J Microencapsul. 1994 Nov-Dec;11(6):627–632. doi: 10.3109/02652049409051112. [DOI] [PubMed] [Google Scholar]