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. 1973 Feb;25(2):287–289. doi: 10.1128/am.25.2.287-289.1973

Simplified Gas Chromatographic Procedure for Identification of Bacterial Metabolic Products

Jan Carlsson 1
PMCID: PMC380789  PMID: 4632854

Abstract

A rapid and simple procedure is described for analysis of fermentation products from anaerobic bacteria grown in glucose broth. A 1-ml sample of the culture is drained through cation-exchange resin in a Pasteur pipette. The effluent fluid is directly analyzed isothermally in a gas chromatograph for volatile fatty acids (C2 to C6) as well as for lactic, pyruvic, and succinic acids. This procedure is considered to be suitable for routine use in clinical bacteriology.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Rogosa M., Love L. L. Direct quantitative gas chromatographic separation of C2-C6 fatty acids, methanol, and ethyl alcohol in aqueous microbial fermentation media. Appl Microbiol. 1968 Feb;16(2):285–290. doi: 10.1128/am.16.2.285-290.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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