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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1979 Feb;76(2):806–808. doi: 10.1073/pnas.76.2.806

Change in nuclear potassium electrochemical activity and puffing of potassium-sensitive salivary chromosome regions during Chironomus development.

P Wuhrmann, H Ineichen, U Riesen-Willi, M Lezzi
PMCID: PMC383057  PMID: 284400

Abstract

Changes in nuclear K+ electrochemical activity and total nuclear K+ content in salivary glands of Chironomus tentans were measured with ion-selective microelectrodes based on valinomycin and with flameless atomic absorption spectrometry, respectively. The K+ activity increased by a factor of 2.6 and the total K+, by a factor of 1.5 as oligopausing larvae developed into prepupae. The extent of decondensation (puffing) of K+-sensitive regions in the polytene chromosomes underwent a parallel increase during this developmental event. In vitro culture of glands from oligopausing larvae resulted in similar changes with respect to nuclear K+ activity and puffing.

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Selected References

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