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. 1979 Feb;76(2):949–953. doi: 10.1073/pnas.76.2.949

Transfection of human lymphoblastoid cells with herpes simplex viral DNA.

G Miller, P Wertheim, G Wilson, J Robinson, J L Geelen, J van der Noordaa, A J van der Eb
PMCID: PMC383102  PMID: 218224

Abstract

The "calcium/dimethyl sulfoxide shock" method of transfection was adapted for use in human lymphoid cell cultures. One microgram of herpes simplex virus type 1 DNA regularly initiated virus replication in four lymphoblastoid cell lines. Per 10(5) cells exposed to 1 microgram of DNA, 0.5--5 cells formed an infectious center. The minimal infective dose of DNA was approximately 500 ng.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dean J. H., Silva J. S., McCOY J. L., Leonard C. M., Cannon G. B., Herberman R. B. Functional activities of rosette separated human peripheral blood leukocytes. J Immunol. 1975 Nov;115(5):1449–1455. [PubMed] [Google Scholar]
  2. Geelen J. L., Walig C., Wertheim P., van der Noordaa J. Human cytomegalovirus DNA. I. Molecular weight and infectivity. J Virol. 1978 Jun;26(3):813–816. doi: 10.1128/jvi.26.3.813-816.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Graham F. L., Veldhuisen G., Wilkie N. M. Infectious herpesvirus DNA. Nat New Biol. 1973 Oct 31;245(148):265–266. doi: 10.1038/newbio245265a0. [DOI] [PubMed] [Google Scholar]
  4. Graham F. L., van der Eb A. J. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 1973 Apr;52(2):456–467. doi: 10.1016/0042-6822(73)90341-3. [DOI] [PubMed] [Google Scholar]
  5. Henderson E., Heston L., Grogan E., Miller G. Radiobiological inactivation of Epstein-Barr virus. J Virol. 1978 Jan;25(1):51–59. doi: 10.1128/jvi.25.1.51-59.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Henle W., Henle G., Zajac B. A., Pearson G., Waubke R., Scriba M. Differential reactivity of human serums with early antigens induced by Epstein-Barr virus. Science. 1970 Jul 10;169(3941):188–190. doi: 10.1126/science.169.3941.188. [DOI] [PubMed] [Google Scholar]
  7. Henle W., Henle G., zur Hausen H. Effect of herpes simplex virus on cultured Burkitt tumor cells and its failure to influence the Epstein-Barr virus carrier state. Cancer Res. 1969 Feb;29(2):489–494. [PubMed] [Google Scholar]
  8. Klein G., Lindahl T., Jondal M., Leibold W., Menézes J., Nilsson K., Sundström C. Continuous lymphoid cell lines with characteristics of B cells (bone-marrow-derived), lacking the Epstein-Barr virus genome and derived from three human lymphomas. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3283–3286. doi: 10.1073/pnas.71.8.3283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Klein G. Studies on the Epstein-Barr Virus genome and the EBV-determined nuclear antigen in human malignant disease. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):783–790. doi: 10.1101/sqb.1974.039.01.090. [DOI] [PubMed] [Google Scholar]
  10. Seigneurin J. M., Desgranges C., Lavoue M. F., de-The G. Herpes simplex-RAJI(A44), a new cell line for serologic testing by immunofluorescence. J Immunol. 1976 Sep;117(3):950–952. [PubMed] [Google Scholar]
  11. Sheldrick P., Laithier M., Lando D., Ryhiner M. L. Infectious DNA from herpes simplex virus: infectivity of double-stranded and single-stranded molecules. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3621–3625. doi: 10.1073/pnas.70.12.3621. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shope T. C., Klein-Robbenhaar J., Miller G. Fatal encephalitis due to Herpesvirus hominis: use of intact brain cells for isolation of virus. J Infect Dis. 1972 May;125(5):542–544. doi: 10.1093/infdis/125.5.542. [DOI] [PubMed] [Google Scholar]
  13. Stow N. D., Wilkie N. M. An improved technique for obtaining enhanced infectivity with herpes simplex virus type 1 DNA. J Gen Virol. 1976 Dec;33(3):447–458. doi: 10.1099/0022-1317-33-3-447. [DOI] [PubMed] [Google Scholar]
  14. Yefenof E., Klein G., Jondal M., Oldstone M. B. Surface markers on human B and T-lymphocytes. IX. Two-color immunofluorescence studies on the association between ebv receptors and complement receptors on the surface of lymphoid cell lines. Int J Cancer. 1976 Jun 15;17(6):693–700. doi: 10.1002/ijc.2910170602. [DOI] [PubMed] [Google Scholar]

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