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. 1983 Aug;80(15):4742–4746. doi: 10.1073/pnas.80.15.4742

Immunocytochemical localization of gamma-glutamyltransferase in induced hyperplastic nodules of rat liver.

H W Spater, N Quintana, F F Becker, A B Novikoff
PMCID: PMC384120  PMID: 6136038

Abstract

The immunocytochemical localization of gamma-glutamyltransferase [(5-glutamyl)-peptide:amino-acid 5-glutamyltransferase, EC 2.3.2.2; gamma-GluTase] was demonstrated in hyperplastic liver induced by the carcinogen 2-acetylaminofluorene (2-AAF). The method used a specific antiserum and protein A-horseradish peroxidase and permitted visualization of antigenic sites at both the light and electron microscopic levels. Electron microscopy revealed deposits of 3,3'-diaminobenzidine (DAB) reaction product in the plasma membranes of (i) hyperplastic cells, (ii) bile canaliculi, (iii) endothelial cell membranes, and (iv) lymphocytes. The so-called ATPase activity was localized in the plasma membrane in bile canaliculi and in endothelial cells; the hyperplastic cells show marked variability in the levels of this activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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