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. 1983 Aug;80(16):5080–5084. doi: 10.1073/pnas.80.16.5080

Localization of a poliovirus type 1 neutralization epitope in viral capsid polypeptide VP1.

S van der Werf, C Wychowski, P Bruneau, B Blondel, R Crainic, F Horodniceanu, M Girard
PMCID: PMC384192  PMID: 6308670

Abstract

Poliovirus type 1 cDNA sequences coding for viral capsid polypeptide VP1 were inserted into the beta-lactamase sequence of Escherichia coli plasmid pBR322. Resulting recombinant plasmid pSW119 expressed in Escherichia coli a VP1-beta-lactamase fusion protein that reacted with antibodies raised against poliovirus capsid polypeptide VP1 and with a monoclonal poliovirus type 1 neutralizing antibody, C3. Deletions of various lengths were generated within the VP1 sequence. The hybrid proteins expressed by the deleted plasmids did not react any more with C3 when the region of VP1 amino acids 95-110 (poliovirus nucleotides 2,754-2,806) was deleted. Therefore, the C3 epitope responsible for virus neutralization is most probably located in this region of the capsid polypeptide.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beatrice S. T., Katze M. G., Zajac B. A., Crowell R. L. Induction of neutralizing antibodies by the coxsackievirus B3 virion polypeptide, VP2. Virology. 1980 Jul 30;104(2):426–438. doi: 10.1016/0042-6822(80)90345-1. [DOI] [PubMed] [Google Scholar]
  2. Bittle J. L., Houghten R. A., Alexander H., Shinnick T. M., Sutcliffe J. G., Lerner R. A., Rowlands D. J., Brown F. Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence. Nature. 1982 Jul 1;298(5869):30–33. doi: 10.1038/298030a0. [DOI] [PubMed] [Google Scholar]
  3. Blondel B., Akacem O., Crainic R., Couillin P., Horodniceanu F. Detection by monoclonal antibodies of an antigenic determinant critical for poliovirus neutralization present on VP1 and on heat-inactivated virions. Virology. 1983 Apr 30;126(2):707–710. doi: 10.1016/s0042-6822(83)80027-0. [DOI] [PubMed] [Google Scholar]
  4. Blondel B., Crainic R. A technique to detect the reaction between poliovirus structural polypeptides and neutralizing anti-polio IgG. Dev Biol Stand. 1981;47:335–337. [PubMed] [Google Scholar]
  5. Blondel B., Crainic R., Horodniceanu F. Le polypeptide structural VP1 du poliovirus type 1 induit des anticorps neutralisants. C R Seances Acad Sci III. 1982 Jan 11;294(2):91–94. [PubMed] [Google Scholar]
  6. Chow M., Baltimore D. Isolated poliovirus capsid protein VP1 induces a neutralizing response in rats. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7518–7521. doi: 10.1073/pnas.79.23.7518. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dorner A. J., Dorner L. F., Larsen G. R., Wimmer E., Anderson C. W. Identification of the initiation site of poliovirus polyprotein synthesis. J Virol. 1982 Jun;42(3):1017–1028. doi: 10.1128/jvi.42.3.1017-1028.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Emini E. A., Dorner A. J., Dorner L. F., Jameson B. A., Wimmer E. Identification of a poliovirus neutralization epitope through use of neutralizing antiserum raised against a purified viral structural protein. Virology. 1983 Jan 15;124(1):144–151. doi: 10.1016/0042-6822(83)90297-0. [DOI] [PubMed] [Google Scholar]
  9. Emini E. A., Jameson B. A., Lewis A. J., Larsen G. R., Wimmer E. Poliovirus neutralization epitopes: analysis and localization with neutralizing monoclonal antibodies. J Virol. 1982 Sep;43(3):997–1005. doi: 10.1128/jvi.43.3.997-1005.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Guo L. H., Wu R. New rapid methods for DNA sequencing based in exonuclease III digestion followed by repair synthesis. Nucleic Acids Res. 1982 Mar 25;10(6):2065–2084. doi: 10.1093/nar/10.6.2065. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hopp T. P., Woods K. R. Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3824–3828. doi: 10.1073/pnas.78.6.3824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Icenogle J., Gilbert S. F., Grieves J., Anderegg J., Rueckert R. A neutralizing monoclonal antibody against poliovirus and its reaction with related antigens. Virology. 1981 Nov;115(1):211–215. doi: 10.1016/0042-6822(81)90103-3. [DOI] [PubMed] [Google Scholar]
  13. Kitamura N., Semler B. L., Rothberg P. G., Larsen G. R., Adler C. J., Dorner A. J., Emini E. A., Hanecak R., Lee J. J., van der Werf S. Primary structure, gene organization and polypeptide expression of poliovirus RNA. Nature. 1981 Jun 18;291(5816):547–553. doi: 10.1038/291547a0. [DOI] [PubMed] [Google Scholar]
  14. Kleid D. G., Yansura D., Small B., Dowbenko D., Moore D. M., Grubman M. J., McKercher P. D., Morgan D. O., Robertson B. H., Bachrach H. L. Cloned viral protein vaccine for foot-and-mouth disease: responses in cattle and swine. Science. 1981 Dec 4;214(4525):1125–1129. doi: 10.1126/science.6272395. [DOI] [PubMed] [Google Scholar]
  15. Laporte J., Grosclaude J., Wantyghem J., Bernard S., Rouzé P. Neutralisation en culture cellulaire du pouvoir infectieuz du virus de la fièvre aphteuse par des sérums provenant de porcs immunisés à l'aide d'une protéine virale purifiée. C R Acad Sci Hebd Seances Acad Sci D. 1973 Jun 18;276(25):3399–3401. [PubMed] [Google Scholar]
  16. Lund G. A., Ziola B. R., Salmi A., Scraba D. G. Structure of the Mengo virion. V. Distribution of the capsid polypeptides with respect to the surface of the virus particle. Virology. 1977 May 1;78(1):35–44. doi: 10.1016/0042-6822(77)90076-9. [DOI] [PubMed] [Google Scholar]
  17. Lusky M., Botchan M. Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences. Nature. 1981 Sep 3;293(5827):79–81. doi: 10.1038/293079a0. [DOI] [PubMed] [Google Scholar]
  18. Makoff A. J., Paynter C. A., Rowlands D. J., Boothroyd J. C. Comparison of the amino acid sequence of the major immunogen from three serotypes of foot and mouth disease virus. Nucleic Acids Res. 1982 Dec 20;10(24):8285–8295. doi: 10.1093/nar/10.24.8285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Minor P. D., Schild G. C., Bootman J., Evans D. M., Ferguson M., Reeve P., Spitz M., Stanway G., Cann A. J., Hauptmann R. Location and primary structure of a major antigenic site for poliovirus neutralization. Nature. 1983 Feb 24;301(5902):674–679. doi: 10.1038/301674a0. [DOI] [PubMed] [Google Scholar]
  20. Murray N. E., Brammar W. J., Murray K. Lambdoid phages that simplify the recovery of in vitro recombinants. Mol Gen Genet. 1977 Jan 7;150(1):53–61. doi: 10.1007/BF02425325. [DOI] [PubMed] [Google Scholar]
  21. Nomoto A., Omata T., Toyoda H., Kuge S., Horie H., Kataoka Y., Genba Y., Nakano Y., Imura N. Complete nucleotide sequence of the attenuated poliovirus Sabin 1 strain genome. Proc Natl Acad Sci U S A. 1982 Oct;79(19):5793–5797. doi: 10.1073/pnas.79.19.5793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Pfaff E., Mussgay M., Böhm H. O., Schulz G. E., Schaller H. Antibodies against a preselected peptide recognize and neutralize foot and mouth disease virus. EMBO J. 1982;1(7):869–874. doi: 10.1002/j.1460-2075.1982.tb01262.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Racaniello V. R., Baltimore D. Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome. Proc Natl Acad Sci U S A. 1981 Aug;78(8):4887–4891. doi: 10.1073/pnas.78.8.4887. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Soberon X., Covarrubias L., Bolivar F. Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325. Gene. 1980 May;9(3-4):287–305. doi: 10.1016/0378-1119(90)90328-o. [DOI] [PubMed] [Google Scholar]
  25. Strohmaier K., Franze R., Adam K. H. Location and characterization of the antigenic portion of the FMDV immunizing protein. J Gen Virol. 1982 Apr;59(Pt 2):295–306. doi: 10.1099/0022-1317-59-2-295. [DOI] [PubMed] [Google Scholar]
  26. Sutcliffe J. G. Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3737–3741. doi: 10.1073/pnas.75.8.3737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wieslander L. A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels. Anal Biochem. 1979 Oct 1;98(2):305–309. doi: 10.1016/0003-2697(79)90145-3. [DOI] [PubMed] [Google Scholar]
  28. van der Werf S., Bregegere F., Kopecka H., Kitamura N., Rothberg P. G., Kourilsky P., Wimmer E., Girard M. Molecular cloning of the genome of poliovirus type 1. Proc Natl Acad Sci U S A. 1981 Oct;78(10):5983–5987. doi: 10.1073/pnas.78.10.5983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. van der Werf S., Dréano M., Bruneau P., Kopecka H., Girard M. Expression of poliovirus capsid polypeptide VP1 in Escherichia coli. Gene. 1983 Jul;23(1):85–93. doi: 10.1016/0378-1119(83)90219-6. [DOI] [PubMed] [Google Scholar]

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