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. 1986 Oct;83(20):7765–7769. doi: 10.1073/pnas.83.20.7765

Rapid and transient rise in diacylglycerol concentration in Daudi cells exposed to interferon.

W H Yap, T S Teo, E McCoy, Y H Tan
PMCID: PMC386802  PMID: 2945205

Abstract

Human beta-interferon stimulates a 4-fold increase in the concentration of diacyglycerol and a 2-fold increase in the concentration of inositol monophosphate in Daudi (human B-lymphoblastoid) cells within 30 sec of exposure of the cells to interferon. The increase in diacylglycerol and in inositol monophosphate is transient and the concentrations of these compounds decrease to basal levels within 10 min. Preincubation of human beta-interferon with anti-interferon antibodies inhibits this effect as well as the binding of interferon to Daudi cells. Diacylglycerol concentrations were unaffected in mouse A9 cells (fibroblasts) incubated with human beta-interferon and in Daudi cells incubated with human gamma-interferon. Mouse A9 cells are insensitive to human interferon and Daudi cells are insensitive to human gamma-interferon. The magnitude of the increase in diacylglycerol concentration stimulated by interferon can be correlated to the interferon-induced inhibition of Daudi cell division in a dose-responsive manner. Phorbol 12-myristate 13-acetate also inhibits Daudi cell division in a dose-responsive manner. It is likely that the sharp and transient increase in diacylglycerol concentration represents one of the early biochemical changes in Daudi cells exposed to interferon.

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Selected References

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  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Berridge M. J., Dawson R. M., Downes C. P., Heslop J. P., Irvine R. F. Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositides. Biochem J. 1983 May 15;212(2):473–482. doi: 10.1042/bj2120473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blackmore P. F., Exton J. H. Assessment of effects of vasopressin, angiotensin II, and glucagon on Ca2+ fluxes and phosphorylase activity in liver. Methods Enzymol. 1985;109:550–558. doi: 10.1016/0076-6879(85)09113-3. [DOI] [PubMed] [Google Scholar]
  4. Branca A. A., Baglioni C. Evidence that types I and II interferons have different receptors. Nature. 1981 Dec 24;294(5843):768–770. doi: 10.1038/294768a0. [DOI] [PubMed] [Google Scholar]
  5. Cheng Y. S., Colonno R. J., Yin F. H. Interferon induction of fibroblast proteins with guanylate binding activity. J Biol Chem. 1983 Jun 25;258(12):7746–7750. [PubMed] [Google Scholar]
  6. Cockcroft S., Gomperts B. D. Role of guanine nucleotide binding protein in the activation of polyphosphoinositide phosphodiesterase. Nature. 1985 Apr 11;314(6011):534–536. doi: 10.1038/314534a0. [DOI] [PubMed] [Google Scholar]
  7. Colby C. B., Inoue M., Thompson M., Tan Y. H. Immunologic differentiation between E. coli and CHO cell-derived recombinant and natural human beta-interferons. J Immunol. 1984 Dec;133(6):3091–3095. [PubMed] [Google Scholar]
  8. Fournier A., Zhang Z. Q., Tan Y. H. Human beta:alpha but not gamma interferon binding site is a product of the chromosome 21 interferon action gene. Somat Cell Mol Genet. 1985 May;11(3):291–295. doi: 10.1007/BF01534686. [DOI] [PubMed] [Google Scholar]
  9. Friedman R. L., Manly S. P., McMahon M., Kerr I. M., Stark G. R. Transcriptional and posttranscriptional regulation of interferon-induced gene expression in human cells. Cell. 1984 Oct;38(3):745–755. doi: 10.1016/0092-8674(84)90270-8. [DOI] [PubMed] [Google Scholar]
  10. Friedman R. L., Stark G. R. alpha-Interferon-induced transcription of HLA and metallothionein genes containing homologous upstream sequences. Nature. 1985 Apr 18;314(6012):637–639. doi: 10.1038/314637a0. [DOI] [PubMed] [Google Scholar]
  11. Habenicht A. J., Glomset J. A., King W. C., Nist C., Mitchell C. D., Ross R. Early changes in phosphatidylinositol and arachidonic acid metabolism in quiescent swiss 3T3 cells stimulated to divide by platelet-derived growth factor. J Biol Chem. 1981 Dec 10;256(23):12329–12335. [PubMed] [Google Scholar]
  12. Hamilton T. A., Becton D. L., Somers S. D., Gray P. W., Adams D. O. Interferon-gamma modulates protein kinase C activity in murine peritoneal macrophages. J Biol Chem. 1985 Feb 10;260(3):1378–1381. [PubMed] [Google Scholar]
  13. Hovanessian A. G. Intracellular events in interferon-treated cells. Differentiation. 1979;15(3):139–151. doi: 10.1111/j.1432-0436.1979.tb01044.x. [DOI] [PubMed] [Google Scholar]
  14. Inoue M., Tan Y. H. Radioimmunoassay for human beta interferon. Infect Immun. 1981 Sep;33(3):763–768. doi: 10.1128/iai.33.3.763-768.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kelly J. M., Gilbert C. S., Stark G. R., Kerr I. M. Differential regulation of interferon-induced mRNAs and c-myc mRNA by alpha- and gamma-interferons. Eur J Biochem. 1985 Dec 2;153(2):367–371. doi: 10.1111/j.1432-1033.1985.tb09312.x. [DOI] [PubMed] [Google Scholar]
  16. Larner A. C., Jonak G., Cheng Y. S., Korant B., Knight E., Darnell J. E., Jr Transcriptional induction of two genes in human cells by beta interferon. Proc Natl Acad Sci U S A. 1984 Nov;81(21):6733–6737. doi: 10.1073/pnas.81.21.6733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lengyel P. Biochemistry of interferons and their actions. Annu Rev Biochem. 1982;51:251–282. doi: 10.1146/annurev.bi.51.070182.001343. [DOI] [PubMed] [Google Scholar]
  18. Nishizuka Y. The role of protein kinase C in cell surface signal transduction and tumour promotion. Nature. 1984 Apr 19;308(5961):693–698. doi: 10.1038/308693a0. [DOI] [PubMed] [Google Scholar]
  19. Okamura H., Berthold W., Hood L., Hunkapiller M., Inoue M., Smith-Johannsen H., Tan Y. H. Human fibroblastoid interferon: immunosorbent column chromatography and N-terminal amino acid sequence. Biochemistry. 1980 Aug 5;19(16):3831–3835. doi: 10.1021/bi00557a028. [DOI] [PubMed] [Google Scholar]
  20. Rashidbaigi A., Langer J. A., Jung V., Jones C., Morse H. G., Tischfield J. A., Trill J. J., Kung H. F., Pestka S. The gene for the human immune interferon receptor is located on chromosome 6. Proc Natl Acad Sci U S A. 1986 Jan;83(2):384–388. doi: 10.1073/pnas.83.2.384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Raziuddin A., Sarkar F. H., Dutkowski R., Shulman L., Ruddle F. H., Gupta S. L. Receptors for human alpha and beta interferon but not for gamma interferon are specified by human chromosome 21. Proc Natl Acad Sci U S A. 1984 Sep;81(17):5504–5508. doi: 10.1073/pnas.81.17.5504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rosa F., Hatat D., Abadie A., Wallach D., Revel M., Fellous M. Differential regulation of HLA-DR mRNAs and cell surface antigens by interferon. EMBO J. 1983;2(9):1585–1589. doi: 10.1002/j.1460-2075.1983.tb01628.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sen G. C. Mechanism of interferon action: progress toward its understanding. Prog Nucleic Acid Res Mol Biol. 1982;27:105–156. doi: 10.1016/s0079-6603(08)60599-1. [DOI] [PubMed] [Google Scholar]
  24. Tan Y. H., Armstrong J. A., Ke Y. H., Ho M. Regulation of cellular interferon production: enhancement by antimetabolites. Proc Natl Acad Sci U S A. 1970 Sep;67(1):464–471. doi: 10.1073/pnas.67.1.464. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tan Y. H. Chromosome 21 and the cell growth inhibitory effect of human interferon preparations. Nature. 1976 Mar 11;260(5547):141–143. doi: 10.1038/260141a0. [DOI] [PubMed] [Google Scholar]
  26. Zhang Z. Q., Fournier A., Tan Y. H. The isolation of human beta-interferon receptor by wheat germ lectin affinity and immunosorbent column chromatographies. J Biol Chem. 1986 Jun 15;261(17):8017–8021. [PubMed] [Google Scholar]

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