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. 1986 Dec;83(23):8863–8867. doi: 10.1073/pnas.83.23.8863

Formation of functional asialoglycoprotein receptor after transfection with cDNAs encoding the receptor proteins.

M McPhaul, P Berg
PMCID: PMC387033  PMID: 3466162

Abstract

The rat asialoglycoprotein receptor (ASGP-R) has been expressed in cultured rat hepatoma cells (HTC cells) after transfection with cloned cDNAs. Fluorescence-activated cell sorting of transfected cells was used to identify the functional cDNA clones and to isolate cells expressing the ASGP-R. Simultaneous or sequential transfections with two cloned cDNAs that encode related but distinctive polypeptide chains were needed to obtain ASGP-R activity; transfection with either cDNA alone failed to produce detectable ASGP-R. The affinity of transduced ASGP-R for asialo orosomucoid is less than that of the native rat ASGP-R, and the number of surface receptors in clones expressing ASGP-R is about one-fifth that found on rat hepatocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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