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. 1986 Dec;83(23):9016–9020. doi: 10.1073/pnas.83.23.9016

MB1, a quail leukocyte-endothelium antigen: partial characterization of the cell surface and secreted forms in cultured endothelial cells.

M C Labastie, T J Poole, B M Péault, N M Le Douarin
PMCID: PMC387065  PMID: 3466174

Abstract

We describe here conditions allowing the selective growth in culture of embryonic capillary endothelial cells from quail yolk sac. Such cultures were set up to characterize an antigen present on the endothelial cell surface and to study whether it was secreted in the culture medium. This antigen, MB1, was previously evidenced by a monoclonal antibody raised to quail IgM heavy chain. It is present at the surface of all endothelial and hemopoietic cells (except mature erythrocytes) starting from the hemangioblast, the early mesodermal precursor of blood and vascular endothelial cells. The MB1 epitope is also found on quail plasma molecules of 80 and 125-200 kDa. By immunoprecipitation of either surface or metabolically labeled endothelial cellular material, we have chemically characterized MB1-bearing components as glycoproteins of apparent molecular mass ranging from 80 to 200 kDa and provided evidence for their release into the culture medium. This is consistent with the hypothesis that, in the quail, vascular endothelium participates in the secretion of the alpha-MB1-positive plasmatic components.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auerbach R., Alby L., Morrissey L. W., Tu M., Joseph J. Expression of organ-specific antigens on capillary endothelial cells. Microvasc Res. 1985 May;29(3):401–411. doi: 10.1016/0026-2862(85)90028-7. [DOI] [PubMed] [Google Scholar]
  2. Banerjee D. K., Ornberg R. L., Youdim M. B., Heldman E., Pollard H. B. Endothelial cells from bovine adrenal medulla develop capillary-like growth patterns in culture. Proc Natl Acad Sci U S A. 1985 Jul;82(14):4702–4706. doi: 10.1073/pnas.82.14.4702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barbu M., Ziller C., Rong P. M., Le Douarin N. M. Heterogeneity in migrating neural crest cells revealed by a monoclonal antibody. J Neurosci. 1986 Aug;6(8):2215–2225. doi: 10.1523/JNEUROSCI.06-08-02215.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Belo M., Martin C., Corbel C., Le Douarin N. M. A novel method to bursectomize avian embryos and obtain quail----chick bursal chimeras. I. Immunocytochemical analysis of such chimeras by using species-specific monoclonal antibodies. J Immunol. 1985 Dec;135(6):3785–3794. [PubMed] [Google Scholar]
  5. Booyse F. M., Sedlak B. J., Rafelson M. E., Jr Culture of arterial endothelial cells: characterization and growth of bovine aortic cells. Thromb Diath Haemorrh. 1975 Dec 15;34(3):825–839. [PubMed] [Google Scholar]
  6. Bowman P. D., Ennis S. R., Rarey K. E., Betz A. L., Goldstein G. W. Brain microvessel endothelial cells in tissue culture: a model for study of blood-brain barrier permeability. Ann Neurol. 1983 Oct;14(4):396–402. doi: 10.1002/ana.410140403. [DOI] [PubMed] [Google Scholar]
  7. Buonassisi V. Sulfated mucopolysaccharide synthesis and secretion in endothelial cell cultures. Exp Cell Res. 1973 Feb;76(2):363–368. doi: 10.1016/0014-4827(73)90388-1. [DOI] [PubMed] [Google Scholar]
  8. Cerveny T. J., Fass D. N., Mann K. G. Synthesis of coagulation factor V by cultured aortic endothelium. Blood. 1984 Jun;63(6):1467–1474. [PubMed] [Google Scholar]
  9. Charron D. J., McDevitt H. O. Characterization of HLA-D-region antigens by two-dimensional gel electrophoresis. Molecular-genotyping. J Exp Med. 1980 Aug 1;152(2 Pt 2):18s–36s. [PubMed] [Google Scholar]
  10. Darnule A. T., Stotzky G., Darnule T. V., Turino G. M., Mandl I. Identification of surface antigens of endothelial cells. Immunol Commun. 1983;12(4):351–362. doi: 10.3109/08820138309050756. [DOI] [PubMed] [Google Scholar]
  11. Folkman J., Haudenschild C. C., Zetter B. R. Long-term culture of capillary endothelial cells. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5217–5221. doi: 10.1073/pnas.76.10.5217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Folkman J., Haudenschild C. Angiogenesis in vitro. Nature. 1980 Dec 11;288(5791):551–556. doi: 10.1038/288551a0. [DOI] [PubMed] [Google Scholar]
  13. Furie M. B., Cramer E. B., Naprstek B. L., Silverstein S. C. Cultured endothelial cell monolayers that restrict the transendothelial passage of macromolecules and electrical current. J Cell Biol. 1984 Mar;98(3):1033–1041. doi: 10.1083/jcb.98.3.1033. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ghandour S., Langley K., Gombos G., Hirn M., Hirsch M. R., Goridis C. A surface marker for murine vascular endothelial cells defined by monoclonal antibody. J Histochem Cytochem. 1982 Feb;30(2):165–170. doi: 10.1177/30.2.7061819. [DOI] [PubMed] [Google Scholar]
  15. Goridis C., Hirsch M., Dossetto M., Baechler E. Identification and characterisation of two surface glycoproteins on cultured cerebellar cells. Brain Res. 1980 Jan 27;182(2):397–414. doi: 10.1016/0006-8993(80)91197-x. [DOI] [PubMed] [Google Scholar]
  16. Gospodarowicz D., Moran J., Braun D., Birdwell C. Clonal growth of bovine vascular endothelial cells: fibroblast growth factor as a survival agent. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4120–4124. doi: 10.1073/pnas.73.11.4120. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Guillemot F. P., Oliver P. D., Peault B. M., Le Douarin N. M. Cells expressing Ia antigens in the avian thymus. J Exp Med. 1984 Dec 1;160(6):1803–1819. doi: 10.1084/jem.160.6.1803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Heifetz A., Johnson A. R., Roberts M. K. Synthesis of lactosaminoglycan-containing glycoproteins by vascular endothelial cells. Biochim Biophys Acta. 1984 Mar 22;798(1):1–7. doi: 10.1016/0304-4165(84)90002-3. [DOI] [PubMed] [Google Scholar]
  19. Hoessli D. C., Vassalli P. High molecular weight surface glycoproteins of murine lymphocytes. J Immunol. 1980 Oct;125(4):1758–1763. [PubMed] [Google Scholar]
  20. Hormia M., Badley R. A., Lehto V. P., Virtanen I. Actomyosin organization in stationary and migrating sheets of cultured human endothelial cells. Exp Cell Res. 1985 Mar;157(1):116–126. doi: 10.1016/0014-4827(85)90156-9. [DOI] [PubMed] [Google Scholar]
  21. Jaffe E. A., Mosher D. F. Synthesis of fibronectin by cultured human endothelial cells. J Exp Med. 1978 Jun 1;147(6):1779–1791. doi: 10.1084/jem.147.6.1779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Jaffe E. A., Nachman R. L., Becker C. G., Minick C. R. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest. 1973 Nov;52(11):2745–2756. doi: 10.1172/JCI107470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Kaplan K. L., Weber D., Cook P., Dalecki M., Rogozinski L., Sepe O., Knowles D., Butler V. P. Monoclonal antibodies to E92, an endothelial cell surface antigen. Arteriosclerosis. 1983 Sep-Oct;3(5):403–412. doi: 10.1161/01.atv.3.5.403. [DOI] [PubMed] [Google Scholar]
  24. Knowles D. M., 2nd, Tolidjian B., Marboe C., D'Agati V., Grimes M., Chess L. Monoclonal anti-human monocyte antibodies OKM1 and OKM5 possess distinctive tissue distributions including differential reactivity with vascular endothelium. J Immunol. 1984 May;132(5):2170–2173. [PubMed] [Google Scholar]
  25. Kramer R. H. Extracellular matrix interactions with the apical surface of vascular endothelial cells. J Cell Sci. 1985 Jun;76:1–16. doi: 10.1242/jcs.76.1.1. [DOI] [PubMed] [Google Scholar]
  26. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  27. Loskutoff D. J., Mussoni L. Interactions between fibrin and the plasminogen activators produced by cultured endothelial cells. Blood. 1983 Jul;62(1):62–68. [PubMed] [Google Scholar]
  28. MacCarthy E. P., Ooi Y. M., Hsu A., Ooi B. S. Evidence for an endothelial cell-derived factor which stimulates the growth of human peripheral blood mononuclear cells. Cell Immunol. 1985 Apr 1;91(2):492–497. doi: 10.1016/0008-8749(85)90246-1. [DOI] [PubMed] [Google Scholar]
  29. Maciag T., Kadish J., Wilkins L., Stemerman M. B., Weinstein R. Organizational behavior of human umbilical vein endothelial cells. J Cell Biol. 1982 Sep;94(3):511–520. doi: 10.1083/jcb.94.3.511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Madri J. A., Pratt B. M. Endothelial cell-matrix interactions: in vitro models of angiogenesis. J Histochem Cytochem. 1986 Jan;34(1):85–91. doi: 10.1177/34.1.2416801. [DOI] [PubMed] [Google Scholar]
  31. Madri J. A., Williams S. K. Capillary endothelial cell cultures: phenotypic modulation by matrix components. J Cell Biol. 1983 Jul;97(1):153–165. doi: 10.1083/jcb.97.1.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. McPherson J., Sage H., Bornstein P. Isolation and characterization of a glycoprotein secreted by aortic endothelial cells in culture. Apparent identity with platelet thrombospondin. J Biol Chem. 1981 Nov 10;256(21):11330–11336. [PubMed] [Google Scholar]
  33. Milici A. J., Furie M. B., Carley W. W. The formation of fenestrations and channels by capillary endothelium in vitro. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6181–6185. doi: 10.1073/pnas.82.18.6181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Miossec P., Cavender D., Ziff M. Production of interleukin 1 by human endothelial cells. J Immunol. 1986 Apr 1;136(7):2486–2491. [PubMed] [Google Scholar]
  35. Peault B. M., Thiery J. P., Le Douarin N. M. Surface marker for hemopoietic and endothelial cell lineages in quail that is defined by a monoclonal antibody. Proc Natl Acad Sci U S A. 1983 May;80(10):2976–2980. doi: 10.1073/pnas.80.10.2976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Picciano P. T., Johnson B., Walenga R. W., Donovan M., Borman B. J., Douglas W. H., Kreutzer D. L. Effects of D-valine on pulmonary artery endothelial cell morphology and function in cell culture. Exp Cell Res. 1984 Mar;151(1):134–147. doi: 10.1016/0014-4827(84)90363-x. [DOI] [PubMed] [Google Scholar]
  37. Pober J. S., Gimbrone M. A., Jr Expression of Ia-like antigens by human vascular endothelial cells is inducible in vitro: demonstration by monoclonal antibody binding and immunoprecipitation. Proc Natl Acad Sci U S A. 1982 Nov;79(21):6641–6645. doi: 10.1073/pnas.79.21.6641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Posnett D. N., Marboe C. C., Knowles D. M., 2nd, Jaffe E. A., Kunkel H. G. A membrane antigen (HC1) selectively present on hairy cell leukemia cells, endothelial cells, and epidermal basal cells. J Immunol. 1984 Jun;132(6):2700–2702. [PubMed] [Google Scholar]
  39. Roska A. K., Johnson A. R., Lipsky P. E. Immunologic function of endothelial cells: guinea pig aortic endothelial cells support mitogen-induced T lymphocyte activation, but do not function as antigen-presenting cells. J Immunol. 1984 Jan;132(1):136–145. [PubMed] [Google Scholar]
  40. Sage H., Johnson C., Bornstein P. Characterization of a novel serum albumin-binding glycoprotein secreted by endothelial cells in culture. J Biol Chem. 1984 Mar 25;259(6):3993–4007. [PubMed] [Google Scholar]
  41. Sariola H., Peault B., LeDouarin N., Buck C., Dieterlen-Lièvre F., Saxén L. Extracellular matrix and capillary ingrowth in interspecies chimeric kidneys. Cell Differ. 1984 Nov;15(1):43–51. doi: 10.1016/0045-6039(84)90028-9. [DOI] [PubMed] [Google Scholar]
  42. Sprengers E. D., Verheijen J. H., Van Hinsbergh V. W., Emeis J. J. Evidence for the presence of two different fibrinolytic inhibitors in human endothelial cell conditioned medium. Biochim Biophys Acta. 1984 Sep 28;801(2):163–170. doi: 10.1016/0304-4165(84)90063-1. [DOI] [PubMed] [Google Scholar]
  43. Stastny P. Endothelial-monocyte antigens. Transplant Proc. 1980 Sep;12(3 Suppl 1):32–36. [PubMed] [Google Scholar]
  44. Sunderland C. A., McMaster W. R., Williams A. F. Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes. Eur J Immunol. 1979 Feb;9(2):155–159. doi: 10.1002/eji.1830090212. [DOI] [PubMed] [Google Scholar]
  45. Thornton S. C., Mueller S. N., Levine E. M. Human endothelial cells: use of heparin in cloning and long-term serial cultivation. Science. 1983 Nov 11;222(4624):623–625. doi: 10.1126/science.6635659. [DOI] [PubMed] [Google Scholar]
  46. Trowbridge I. S. Interspecies spleen-myeloma hybrid producing monoclonal antibodies against mouse lymphocyte surface glycoprotein, T200. J Exp Med. 1978 Jul 1;148(1):313–323. doi: 10.1084/jem.148.1.313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. Trowbridge I. S., Mazauskas C. Immunological properties of murine thymus-dependent lymphocyte surface glycoproteins. Eur J Immunol. 1976 Aug;6(8):557–562. doi: 10.1002/eji.1830060806. [DOI] [PubMed] [Google Scholar]
  48. Trowbridge I. S., Ralph P., Bevan M. J. Differences in the surface proteins of mouse B and T cells. Proc Natl Acad Sci U S A. 1975 Jan;72(1):157–161. doi: 10.1073/pnas.72.1.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  49. Tulsiani D. R., Harris T. M., Touster O. Swainsonine inhibits the biosynthesis of complex glycoproteins by inhibition of Golgi mannosidase II. J Biol Chem. 1982 Jul 25;257(14):7936–7939. [PubMed] [Google Scholar]
  50. Voyta J. C., Via D. P., Butterfield C. E., Zetter B. R. Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein. J Cell Biol. 1984 Dec;99(6):2034–2040. doi: 10.1083/jcb.99.6.2034. [DOI] [PMC free article] [PubMed] [Google Scholar]
  51. Watt S. L., Auerbach R. A mitogenic factor for endothelial cells obtained from mouse secondary mixed leukocyte cultures. J Immunol. 1986 Jan;136(1):197–202. [PubMed] [Google Scholar]
  52. Weksler B. B., Marcus A. J., Jaffe E. A. Synthesis of prostaglandin I2 (prostacyclin) by cultured human and bovine endothelial cells. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3922–3926. doi: 10.1073/pnas.74.9.3922. [DOI] [PMC free article] [PubMed] [Google Scholar]

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