Abstract
The in vitro conversion of single-stranded DNA from bacteriophage fd to duplex structures depends on E. coli RNA polymerase, DNA polymerase III, riboand deoxyribonucleoside triphosphates, Mg+2, spermidine or DNA-unwinding protein of E. coli, and two additional protein factors, referred to here as Factors I and II. These two factors are also essential for dTMP incorporation catalyzed by DNA polymerase III and dependent on poly(dA)·oligo(dT) primer-template. In the latter reaction, there is an absolute dependency on ATP or dATP.
Keywords: DNA replication, fd DNA, poly(dA)·oligo(dT)
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