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. 1974 Jan;71(1):144–147. doi: 10.1073/pnas.71.1.144

Indirect Ultraviolet-Reactivation of Phage λ

Jacqueline George 1,*, Raymond Devoret 1, Miroslav Radman 1,†
PMCID: PMC387953  PMID: 4589889

Abstract

When an F- recipient Escherichia coli K12 bacterium receives Hfr or F-lac+ DNA from an ultraviolet-irradiated donor, its capacity to promote DNA repair and mutagenesis of ultraviolet-damaged phage λ is substantially increased.

We call this phenomenon indirect ultraviolet-reactivation, since its features are essentially the same as those of ultraviolet-reactivation; this repair process occurs in pyrimidine dimer excision-deficient strains and produces clear plaque mutations of the restored phage. Moreover, this process is similar to indirect ultraviolet-induction of prophage λ, since it is promoted by conjugation. However, contrarily to indirect induction, it is produced by Hfr donors and occurs in recipients restricting the incoming ultraviolet-damaged donor DNA.

The occurrence of indirect ultraviolet-reactivation provides evidence for the existence in E. coli of an inducible error-prone mechanism for the repair of DNA.

Keywords: mutagenesis, repair of DNA, bacterial conjugation, Escherichia coli

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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