Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1974 Feb;71(2):446–450. doi: 10.1073/pnas.71.2.446

In Vitro Synthesis of Ribosomal Proteins Directed by Escherichia coli DNA*

E Kaltschmidt 1,2,3, L Kahan 1,2,3, M Nomura 1,2,3
PMCID: PMC388023  PMID: 4205593

Abstract

In vitro synthesis of a number of E. coli 30S ribosomal proteins has been demonstrated in a cell-free system consisting of ribosomes, initiation factors, RNA polymerase, a fraction containing soluble enzymes and factors, and E. coli DNA. DNA-dependent synthesis of the following 30S proteins has been demonstrated: S4, S5, S7, S8, S9, S10, S13, S14, S16, S19, and S20.

Keywords: ribosome reconstitution, radio-immunodiffusion, protein identification

Full text

PDF
450

Images in this article

448Image
on p.448
448Image
on p.448
449Image
on p.449

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burgess R. R. A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase. J Biol Chem. 1969 Nov 25;244(22):6160–6167. [PubMed] [Google Scholar]
  2. Cashel M., Gallant J. Two compounds implicated in the function of the RC gene of Escherichia coli. Nature. 1969 Mar 1;221(5183):838–841. doi: 10.1038/221838a0. [DOI] [PubMed] [Google Scholar]
  3. Chambers D. A., Zubay G. The stimulatory effect of cyclic adenosine 3'5'-monophosphate on DNA-directed synthesis of beta-galactosidase in a cell-free system. Proc Natl Acad Sci U S A. 1969 May;63(1):118–122. doi: 10.1073/pnas.63.1.118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Crawford L. V., Gesteland R. F. Synthesis of polyoma proteins in vitro. J Mol Biol. 1973 Mar 15;74(4):627–634. doi: 10.1016/0022-2836(73)90053-3. [DOI] [PubMed] [Google Scholar]
  5. Davies J., Nomura M. The genetics of bacterial ribosomes. Annu Rev Genet. 1972;6:203–234. doi: 10.1146/annurev.ge.06.120172.001223. [DOI] [PubMed] [Google Scholar]
  6. Gold L. M., Schweiger M. Synthesis of phage-specific alpha- and beta-glucosyl transferases directed by T-even DNA in vitro. Proc Natl Acad Sci U S A. 1969 Mar;62(3):892–898. doi: 10.1073/pnas.62.3.892. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Higo K., Held W., Kahan L., Nomura M. Functional correspondence between 30S ribosomal proteins of Escherichia coli and Bacillus stearothermophilus. Proc Natl Acad Sci U S A. 1973 Mar;70(3):944–948. doi: 10.1073/pnas.70.3.944. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kahan L., Zengel J., Nomura M., Bollen A., Herzog A. The structural gene for the ribosomal protein S18 in Escherichia coli. II. Chemical studies on the protein S18 having an altered electrophoretic mobility. J Mol Biol. 1973 Jun 5;76(4):473–483. doi: 10.1016/0022-2836(73)90486-5. [DOI] [PubMed] [Google Scholar]
  9. Kaltschmidt E., Dzionara M., Wittmann H. G. Ribosomal proteins. XV. Amino acid compositions of isolated ribosomal proteins from 30S and 50S subunits of Escherichia coli. Mol Gen Genet. 1970;109(4):292–297. doi: 10.1007/BF00267698. [DOI] [PubMed] [Google Scholar]
  10. LEBOY P. S., COX E. C., FLAKS J. G. THE CHROMOSOMAL SITE SPECIFYING A RIBOSOMAL PROTEIN IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1964 Dec;52:1367–1374. doi: 10.1073/pnas.52.6.1367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mizushima S., Nomura M. Assembly mapping of 30S ribosomal proteins from E. coli. Nature. 1970 Jun 27;226(5252):1214–1214. doi: 10.1038/2261214a0. [DOI] [PubMed] [Google Scholar]
  12. Muto A. Messenger activity of nascent ribosomal RNA. J Mol Biol. 1968 Aug 28;36(1):1–14. doi: 10.1016/0022-2836(68)90214-3. [DOI] [PubMed] [Google Scholar]
  13. Nomura M. Assembly of bacterial ribosomes. Science. 1973 Mar 2;179(4076):864–873. doi: 10.1126/science.179.4076.864. [DOI] [PubMed] [Google Scholar]
  14. Nomura M., Mizushima S., Ozaki M., Traub P., Lowry C. V. Structure and function of ribosomes and their molecular components. Cold Spring Harb Symp Quant Biol. 1969;34:49–61. doi: 10.1101/sqb.1969.034.01.009. [DOI] [PubMed] [Google Scholar]
  15. Reiner A. M. Characterization of polynucleotide phosphorylase mutants of Escherichia coli. J Bacteriol. 1969 Mar;97(3):1437–1443. doi: 10.1128/jb.97.3.1437-1443.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Traub P., Nomura M. Structure and function of E. coli ribosomes. V. Reconstitution of functionally active 30S ribosomal particles from RNA and proteins. Proc Natl Acad Sci U S A. 1968 Mar;59(3):777–784. doi: 10.1073/pnas.59.3.777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Travers A. Control of ribosomal RNA synthesis in vitro. Nature. 1973 Jul 6;244(5410):15–18. doi: 10.1038/244015a0. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES