Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1974 Mar;71(3):598–601. doi: 10.1073/pnas.71.3.598

Comparison of Weigert Stained Sections with Unfixed, Unstained Sections for Study of Myelin Sheaths

Curt P Richter 1, Carolyn L Warner 1
PMCID: PMC388058  PMID: 4132530

Abstract

Myelinated fibers can be demonstrated in thin unstained brain sections of freshly killed uninjected animals. With this method myelinated fibers stand out as clearly as in stained Weigert sections from formalin-fixed brains. Unstained unfixed sections can be viewed under ordinary light within minutes after death of the animal and immediately photographed for permanent records. Such sections have value for neuroanatomical studies, and for rapid localization of various types of experimental lesions.

Full text

PDF
600

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Hutchinson R. R., Renfrew J. W. A simple histological technique for localizing electrode tracks and lesions within the brain. J Exp Anal Behav. 1967 May;10(3):277–280. doi: 10.1901/jeab.1967.10-277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. POWELL E. W. A RAPID METHOD OF INTRACRANIAL ELECTRODE LOCALIZATION USING UNSTAINED FROZEN SECTIONS. Electroencephalogr Clin Neurophysiol. 1964 Oct;17:432–434. doi: 10.1016/0013-4694(64)90168-3. [DOI] [PubMed] [Google Scholar]
  3. SHANKLIN W. M., ISSIDORIDES M., SALAM M. Histochemistry of the cerebral cortex from a case of amaurotic family idiocy. J Neuropathol Exp Neurol. 1962 Apr;21:284–293. doi: 10.1097/00005072-196204000-00009. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES