Abstract
The envelope of the spinach chloroplast is a yellow membrane system with a unique carotenoid composition. Envelopes prepared from dark-treated leaves had a violaxanthin content up to 3.5 times the lutein plus zeaxanthin content, whereas in chloroplast envelopes from illuminated leaves this ratio was only 0.75. Light-catalyzed changes in violaxanthin content also occurred in the thylakoid fraction.
The role of this reversible light-catalyzed de-epoxidation of violaxanthin in the function of the envelope of the chloroplast is discussed.
Keywords: spinach, light-catalyzed changes
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Douce R., Holtz R. B., Benson A. A. Isolation and properties of the envelope of spinach chloroplasts. J Biol Chem. 1973 Oct 25;248(20):7215–7222. [PubMed] [Google Scholar]
- Hager A., Stransky H. Das Carotinoidmuster und die Verbreitung des lichtinduzierten Xanthophyllcyclus in verschiedenen Algenklassen. 3. Grünalgen. Arch Mikrobiol. 1970;72(1):68–83. [PubMed] [Google Scholar]
- Hager A., Stransky H. Das Carotinoidmuster und die Verbreitung des lichtinduzierten Xanthophyllcyclus in verschiedenen Algenklassen. I. Methoden zur Identifizierung der Pigmente. Arch Mikrobiol. 1970;71(2):132–163. [PubMed] [Google Scholar]
- Jeffrey S. W. Quantitative thin-layer chromatography of chlorophylls and carotenoids from marine algae. Biochim Biophys Acta. 1968 Aug 20;162(2):271–285. doi: 10.1016/0005-2728(68)90109-6. [DOI] [PubMed] [Google Scholar]
- KRINSKY N. I. CAROTENOID DE-EPOXIDATIONS IN ALGAE. I. PHOTOCHEMICAL TRANSFORMATION OF ANTHERAXANTHIN TO ZEAXANTHIN. Biochim Biophys Acta. 1964 Nov 29;88:487–491. [PubMed] [Google Scholar]
- LEECH R. M. THE ISOLATION OF STRUCTURALLY INTACT CHLOROPLASTS. Biochim Biophys Acta. 1964 May 25;79:637–639. doi: 10.1016/0926-6577(64)90235-9. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- YAMAMOTO H. Y., NAKAYAMA T. O., CHICHESTER C. O. Studies on the light and dark interconversions of leaf xanthophylls. Arch Biochem Biophys. 1962 Apr;97:168–173. doi: 10.1016/0003-9861(62)90060-7. [DOI] [PubMed] [Google Scholar]
- Yamamoto H. Y., Chang J. L., Aihara M. S. Light-induced interconversion of violaxanthin and zeaxanthin in New Zealand spinach-leaf segments. Biochim Biophys Acta. 1967 Jul 25;141(2):342–347. doi: 10.1016/0304-4165(67)90108-0. [DOI] [PubMed] [Google Scholar]
- Yamamoto H. Y., Kamite L., Wang Y. Y. An Ascorbate-induced Absorbance Change in Chloroplasts from Violaxanthin De-epoxidation. Plant Physiol. 1972 Feb;49(2):224–228. doi: 10.1104/pp.49.2.224. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yamamoto H. Y., Wang Y., Kamite L. A chloroplast absorbance change from violaxanthin de-epoxidation. A possible component of 515 nm changes. Biochem Biophys Res Commun. 1971 Jan 8;42(1):37–42. doi: 10.1016/0006-291x(71)90358-5. [DOI] [PubMed] [Google Scholar]