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. 1974 Apr;71(4):1021–1025. doi: 10.1073/pnas.71.4.1021

Activity of Colicin E3-Treated Ribosomes in Initiation and in Chain Elongation

Phang-Cheng Tai 1, Bernard D Davis 1
PMCID: PMC388153  PMID: 4598289

Abstract

Colicin E3 (called E3 here) is shown to act on polysomal ribosomes as well as on free ribosomes. This treatment severely inhibits subsequent chain elongation, both on preformed polysomes completing their nascent chains and on ribosomes translating poly(U). Elevated Mg++ concentration relieves this inhibition. However, elevated Mg++ concentration does not relieve the inhibition of ribosomes initiating on natural messenger; hence damage by E3 appears to have an additional, specific effect on some stage in initiation.

The effect of damage by E3 resembles that of streptomycin in several respects. This similarity suggests that the two agents may act on closely related sites on the ribosome.

Keywords: purified polysomes, immunity substance, streptomycin

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boon T. Inactivation of ribosomes in vitro by colicin E 3 . Proc Natl Acad Sci U S A. 1971 Oct;68(10):2421–2425. doi: 10.1073/pnas.68.10.2421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boon T. Inactivation of ribosomes in vitro by colicin E 3 and its mechanism of action. Proc Natl Acad Sci U S A. 1972 Mar;69(3):549–552. doi: 10.1073/pnas.69.3.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bowman C. M., Dahlberg J. E., Ikemura T., Konisky J., Nomura M. Specific inactivation of 16S ribosomal RNA induced by colicin E3 in vivo. Proc Natl Acad Sci U S A. 1971 May;68(5):964–968. doi: 10.1073/pnas.68.5.964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bowman C. M. Inactivation of ribosomes by colicin E3 in vitro: Requirement for 50 S ribosomal subunits. FEBS Lett. 1972 Apr 15;22(1):73–75. doi: 10.1016/0014-5793(72)80222-9. [DOI] [PubMed] [Google Scholar]
  5. Bowman C. M., Sidikaro J., Nomura M. Specific inactivation of ribosomes by colicin E3 in vitro and mechanism of immunity in colicinogenic cells. Nat New Biol. 1971 Dec 1;234(48):133–137. doi: 10.1038/newbio234133a0. [DOI] [PubMed] [Google Scholar]
  6. Cammack K. A., Wade H. E. The sedimentation behaviour of ribonuclease-active and -inactive ribosomes from bacteria. Biochem J. 1965 Sep;96(3):671–680. doi: 10.1042/bj0960671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chang F. N., Flaks J. G. Binding of dihydrostreptomycin to Escherichia coli ribosomes: characteristics and equilibrium of the reaction. Antimicrob Agents Chemother. 1972 Oct;2(4):294–307. doi: 10.1128/aac.2.4.294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DAVIES J., GILBERT W., GORINI L. STREPTOMYCIN, SUPPRESSION, AND THE CODE. Proc Natl Acad Sci U S A. 1964 May;51:883–890. doi: 10.1073/pnas.51.5.883. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dahlberg A. E., Lund E., Kjeldgaard N. O., Bowman C. M., Nomura M. Colicin E3 induced cleavage of 16S ribosomal ribonucleic acid; blocking effects of certain antibiotics. Biochemistry. 1973 Feb 27;12(5):948–950. doi: 10.1021/bi00729a025. [DOI] [PubMed] [Google Scholar]
  10. Davies J., Gorini L., Davis B. D. Misreading of RNA codewords induced by aminoglycoside antibiotics. Mol Pharmacol. 1965 Jul;1(1):93–106. [PubMed] [Google Scholar]
  11. Kang S. S. A mutant of Escherichia coli with temperature-sensitive streptomycin protein. Proc Natl Acad Sci U S A. 1970 Mar;65(3):544–550. doi: 10.1073/pnas.65.3.544. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kang S. S. Temperature sensitive alteration of 30S subunits demonstrated by in vitro reassociation of functional ribosomes. Nature. 1970 Mar 21;225(5238):1132–1133. doi: 10.1038/2251132a0. [DOI] [PubMed] [Google Scholar]
  13. Kaufmann Y., Zamir A. Protection of E. coli ribosomes against colicin E3-induced inactivation by bound aminoacyl-tRNA. FEBS Lett. 1973 Nov 1;36(3):277–280. doi: 10.1016/0014-5793(73)80390-4. [DOI] [PubMed] [Google Scholar]
  14. Konisky J., Nomura M. Interaction of colicins with bacterial cells. II. Specific alteration of Escherichia coli ribosomes induced by colicin E3 in vivo. J Mol Biol. 1967 Jun 14;26(2):181–195. doi: 10.1016/0022-2836(67)90290-2. [DOI] [PubMed] [Google Scholar]
  15. Lelong J. C., Gros D., Cousin M. A., Grunberg-Manago M., Gros F. Streptomycin induced release of fMet-tRNA from the ribosomal initiation complex. Biochem Biophys Res Commun. 1971 Feb 5;42(3):530–537. doi: 10.1016/0006-291x(71)90403-7. [DOI] [PubMed] [Google Scholar]
  16. Luzzatto L., Apirion D., Schlessinger D. Streptomycin action: greater inhibition of Escherichia coli ribosome function with exogenous than with endogenous messenger ribonucleic acid. J Bacteriol. 1969 Jul;99(1):206–209. doi: 10.1128/jb.99.1.206-209.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Miskin R., Zamir A. Effect of streptomycin on ribosome interconversion, a possible basis for the action of the antibiotic. Nat New Biol. 1972 Jul 19;238(81):78–80. doi: 10.1038/newbio238078a0. [DOI] [PubMed] [Google Scholar]
  18. Modolell J., Davis B. D. Breakdown by streptomycin of initiation complexes formed on ribosomes of Escherichia coli. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1148–1155. doi: 10.1073/pnas.67.3.1148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ozaki M., Mizushima S., Nomura M. Identification and functional characterization of the protein controlled by the streptomycin-resistant locus in E. coli. Nature. 1969 Apr 26;222(5191):333–339. doi: 10.1038/222333a0. [DOI] [PubMed] [Google Scholar]
  20. Senior B. W., Holland I. B. Effect of colicin E3 upon the 30S ribosomal subunit of Escherichia coli. Proc Natl Acad Sci U S A. 1971 May;68(5):959–963. doi: 10.1073/pnas.68.5.959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Senior B. W., Kwasniak J., Holland I. B. Colicin E3-directed changes in ribosome function and polyribosome metabolism in Escherichia coli K12. J Mol Biol. 1970 Oct 28;53(2):205–220. doi: 10.1016/0022-2836(70)90295-0. [DOI] [PubMed] [Google Scholar]
  22. Sidikaro J., Nomura M. Colicin E3-induced in vitro inactivation of ribosomes from colicin-insensitive bacterial species. FEBS Lett. 1973 Jan 1;29(1):15–19. doi: 10.1016/0014-5793(73)80005-5. [DOI] [PubMed] [Google Scholar]
  23. Tai P. C., Wallace B. J., Herzog E. L., Davis B. D. Properties of initiation-free polysomes of Escherichia coli. Biochemistry. 1973 Feb;12(4):609–615. doi: 10.1021/bi00728a007. [DOI] [PubMed] [Google Scholar]
  24. Wallace B. J., Tai P. C., Davis B. D. Effect of streptomycin on the response of Escherichia coli ribosomes to the dissociation factor. J Mol Biol. 1973 Apr 5;75(2):391–400. doi: 10.1016/0022-2836(73)90029-6. [DOI] [PubMed] [Google Scholar]
  25. Wallace B. J., Tai P. C., Herzog E. L., Davis B. D. Partial inhibition of polysomal ribosomes of Escherichia coli by streptomycin. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1234–1237. doi: 10.1073/pnas.70.4.1234. [DOI] [PMC free article] [PubMed] [Google Scholar]

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