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. 1974 Apr;71(4):1169–1173. doi: 10.1073/pnas.71.4.1169

Recombinant Rabbit Secretory Immunoglobulin Molecules: Alpha Chains with Maternal (Paternal) Variable-Region Allotypes and Paternal (Maternal) Constant-Region Allotypes

Katherine L Knight 1,2, Thomas R Malek 1,2, W Carey Hanly 1,2
PMCID: PMC388185  PMID: 4133846

Abstract

A population of IgA molecules having heavy chains coded by two parental chromosomes in trans position has been identified in rabbits heterozygous at both the VHa locus, which controls allotypic specificities on the variable part of heavy chains, and the Cαg locus, which controls allotypic specificities on the constant part of alpha chains. These recombinant molecules have alpha-chain allotypic specificities controlled by both the maternal VHa gene and the paternal Cαg gene or conversely, the paternal VHa gene and the maternal Cαg gene. These recombinant molecules were found in F(ab)2α fractions obtained after passage of F(ab)2α preparations through immunosorbent columns designed to remove one population of F(ab)2α molecules, i.e., g74- or g75-type molecules. The effluent F(ab)2α fractions were then examined by radioprecipitation methods for allotypic specificities controlled by the VHa and Cαg loci. About 40% of the g75 F(ab)2α molecules from each of three rabbits with the a1g74 and a2g75 allogroups were alg75 recombinants. These alg75 recombinant molecules represented from 2.5-5.6% of the total unfractionated F(ab)2α sample. The F(ab)2α fractions from two rabbits with the a1g75 and a3g74 allogroups had from 1.8-8.2% recombinant molecules: some were alg74 recombinants and some were a3g75 recombinants. Somatic recombination as a mechanism responsible for the synthesis of polypeptide chains in which part of the information is obtained from one chromosome and part from the homologous chromosome is discussed.

Keywords: somatic recombination, immunosorbents, F(ab)2α fragments

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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