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. 1974 Apr;71(4):1255–1259. doi: 10.1073/pnas.71.4.1255

Isolation of Heterogeneous Circular DNA from Induced Lysogens of Bacteriophage Mu-1

Barbara T Waggoner 1, Nélida S González 1,*, Austin L Taylor 1
PMCID: PMC388204  PMID: 4598297

Abstract

Covalently-closed circular DNA molecules are formed after induction of phage Mu cts4 and after infection with phage Mu cts4. The circular molecules obtained after induction have a molecular length range from 36.5 to 156.7 kilobases as measured by electron microscopic techniques. These heterogeneous molecules have no consistent correlation to exact multiples of a Mu genome equivalent (37.3 ± 1.2 kilobases). Direct evidence is given that these molecules contain phage Mu DNA that is covalently linked to other DNA sequences.

Keywords: cesium chloride-ethidium bromide gradients, electron microscopy, heat-inducible mutants

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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