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. 1974 Apr;71(4):1511–1515. doi: 10.1073/pnas.71.4.1511

Novel Properties of Satellite DNA from Muskmelon

Arnold J Bendich 1,2, Robert S Anderson 1,2
PMCID: PMC388260  PMID: 4524654

Abstract

The purified dense satellite of muskmelon (Cucumis melo L.) represents 30% of the total DNA and forms a sharp, unimodal peak in both neutral and alkaline CsCl gradients. Spectrophotometric melting and DNA reassociation analyses revealed that one-third of the satellite is high melting and has a complexity of about 2.5 × 105 daltons, while the remaining two-thirds of the satellite melts 8° lower and has a complexity of about 5 × 108 daltons. The thermal stability of reassociated satellite DNA indicates that the multiple copies of the two melting fractions are essentially identical. The sharp, unimodal peak in Cs2SO4 becomes two distinct peaks when either silver or mercuric ions are included in the Cs2SO4 gradient

Keywords: melting curves, CsCl gradients, Ag+-Cs2SO4 gradients

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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