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. 1974 Jun;71(6):2188–2192. doi: 10.1073/pnas.71.6.2188

Transcription of Saccharomyces cerevisiae Ribosomal DNA In Vivo and In Vitro

Jane Harris Cramer *, Jesus Sebastian †, Robert H Rownd *, Harlyn O Halvorson †
PMCID: PMC388416  PMID: 4601581

Abstract

In vivo, ribosomal RNA of Saccharomyces cerevisiae is transcribed from the light strand of γ DNA. In vitro, γ DNA is transcribed with equal efficiency by both of the yeast nuclear RNA polymerases, polymerase I and polymerase II; however the RNA products synthesized by the two enzymes differ from each other both in size and in the relative composition of guanine and cytosine. RNA synthesized by polymerase I hybridizes preferentially to the light strand, while that synthesized by polymerase II hybridizes equally well to either strand. Selective transcription of the light strand of the ribosomal DNA also occurs when high-molecular-weight total nuclear DNA is used as template.

Keywords: asymmetric transcription, yeast RNA polymerase, ribosomal RNA, DNA·RNA hybridization

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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