Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1974 Jun;71(6):2188–2192. doi: 10.1073/pnas.71.6.2188

Transcription of Saccharomyces cerevisiae Ribosomal DNA In Vivo and In Vitro

Jane Harris Cramer *, Jesus Sebastian †, Robert H Rownd *, Harlyn O Halvorson †
PMCID: PMC388416  PMID: 4601581

Abstract

In vivo, ribosomal RNA of Saccharomyces cerevisiae is transcribed from the light strand of γ DNA. In vitro, γ DNA is transcribed with equal efficiency by both of the yeast nuclear RNA polymerases, polymerase I and polymerase II; however the RNA products synthesized by the two enzymes differ from each other both in size and in the relative composition of guanine and cytosine. RNA synthesized by polymerase I hybridizes preferentially to the light strand, while that synthesized by polymerase II hybridizes equally well to either strand. Selective transcription of the light strand of the ribosomal DNA also occurs when high-molecular-weight total nuclear DNA is used as template.

Keywords: asymmetric transcription, yeast RNA polymerase, ribosomal RNA, DNA·RNA hybridization

Full text

PDF
2192

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bhargava M. M., Cramer J. H., Halvorson H. O. Isolation of high molecular weight DNA from yeast nuclei. Anal Biochem. 1972 Sep;49(1):276–284. doi: 10.1016/0003-2697(72)90266-7. [DOI] [PubMed] [Google Scholar]
  2. Cramer J. H., Bhargava M. M., Halvorson H. O. Isolation and characterization of DNA of Saccharomyces cerevisiae. J Mol Biol. 1972 Oct 28;71(1):11–20. doi: 10.1016/0022-2836(72)90396-8. [DOI] [PubMed] [Google Scholar]
  3. Dezélée S., Sentenac A. Role of DNA-RNA hybrids in eukaryotes. Purification and properties of yeast RNA polymerase B. Eur J Biochem. 1973 Apr 2;34(1):41–52. doi: 10.1111/j.1432-1033.1973.tb02726.x. [DOI] [PubMed] [Google Scholar]
  4. Fauman M., Rabiwitz M., Getz G. S. Base composition and sedimentation properties of mitochondrial RNA of Saccharomyces cerebisiae. Biochim Biophys Acta. 1969 Jun 17;182(2):355–360. doi: 10.1016/0005-2787(69)90186-5. [DOI] [PubMed] [Google Scholar]
  5. Goldberg S., Oyen T., Idriss J. M., Halvorson H. O. Use of disomic strains to study the arrangement of ribosomal cistrons in saccharomyces of ribosomal cistrons in Saccharomyces cerevisiae. Mol Gen Genet. 1972;116(2):139–157. doi: 10.1007/BF00582223. [DOI] [PubMed] [Google Scholar]
  6. Hecht R. M., Birnstiel M. L. Integrity of the DNA template, a prerequisite for the faithful transcription of Xenopus rDNA in vitro. Eur J Biochem. 1972 Sep 25;29(3):489–499. doi: 10.1111/j.1432-1033.1972.tb02013.x. [DOI] [PubMed] [Google Scholar]
  7. Kaback D. B., Bhargava M. M., Halvorson H. O. Letter: Location and arrangement of genes coding for ribosomal RNA in Saccharomyces cerevisiae. J Mol Biol. 1973 Oct 5;79(4):735–739. doi: 10.1016/0022-2836(73)90076-4. [DOI] [PubMed] [Google Scholar]
  8. Meilhac M., Chambon P. Animal DNA-dependent RNA polymerases. Initiation sites on calf-thymus DNA. Eur J Biochem. 1973 Jun 15;35(3):454–463. doi: 10.1111/j.1432-1033.1973.tb02859.x. [DOI] [PubMed] [Google Scholar]
  9. Reeder R. H., Roeder R. G. Ribosomal RNA synthesis in isolated nuclei. J Mol Biol. 1972 Jun 28;67(3):433–441. doi: 10.1016/0022-2836(72)90461-5. [DOI] [PubMed] [Google Scholar]
  10. Reeder R. H., Roeder R. G. Ribosomal RNA synthesis in isolated nuclei. J Mol Biol. 1972 Jun 28;67(3):433–441. doi: 10.1016/0022-2836(72)90461-5. [DOI] [PubMed] [Google Scholar]
  11. Retèl J., Planta R. J. Nuclear satellite DNAs of yeast. Biochim Biophys Acta. 1972 Oct 27;281(3):299–309. doi: 10.1016/0005-2787(72)90442-x. [DOI] [PubMed] [Google Scholar]
  12. Roeder R. G., Rutter W. J. Specific nucleolar and nucleoplasmic RNA polymerases. Proc Natl Acad Sci U S A. 1970 Mar;65(3):675–682. doi: 10.1073/pnas.65.3.675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schweizer E., MacKechnie C., Halvorson H. O. The redundancy of ribosomal and transfer RNA genes in Saccharomyces cerevisiae. J Mol Biol. 1969 Mar 14;40(2):261–277. doi: 10.1016/0022-2836(69)90474-4. [DOI] [PubMed] [Google Scholar]
  14. Sebastian J., Bhargava M. M., Halvorson H. O. Nuclear deoxyribonucleic acid-dependent ribonucleic acid polymerases from Saccharomyces cerevisiae. J Bacteriol. 1973 Apr;114(1):1–6. doi: 10.1128/jb.114.1.1-6.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sebastian J., Mian F., Halvorson H. O. Effect of the growth rate on the level of the DNA-dependent RNA polymerases in Saccharomyces cerevisiae. FEBS Lett. 1973 Aug 15;34(2):159–162. doi: 10.1016/0014-5793(73)80782-3. [DOI] [PubMed] [Google Scholar]
  16. Smuckler E. A., Tata J. R. Nearest neighbour base frequency of the RNA formed by rat liver DNA-dependent RNA polymerase A and B with homologous DNA. Biochem Biophys Res Commun. 1972 Oct 6;49(1):16–22. doi: 10.1016/0006-291x(72)90003-4. [DOI] [PubMed] [Google Scholar]
  17. Sogin S. J., Haber J. E., Halvorson H. O. Relationship between sporulation-specific 20S ribonucleic acid and ribosomal ribonucleic acid processing in Saccharomyces cerevisiae. J Bacteriol. 1972 Nov;112(2):806–814. doi: 10.1128/jb.112.2.806-814.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Udem S. A., Warner J. R. Ribosomal RNA synthesis in Saccharomyces cerevisiae. J Mol Biol. 1972 Mar 28;65(2):227–242. doi: 10.1016/0022-2836(72)90279-3. [DOI] [PubMed] [Google Scholar]
  19. VINOGRAD J., MORRIS J., DAVIDSON N., DOVE W. F., Jr The bouyant behavior of viral and bacterial DNA in alkaline CsCl. Proc Natl Acad Sci U S A. 1963 Jan 15;49:12–17. doi: 10.1073/pnas.49.1.12. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Warner J. R. The assembly of ribosomes in yeast. J Biol Chem. 1971 Jan 25;246(2):447–454. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES