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. 1974 Jun;71(6):2515–2519. doi: 10.1073/pnas.71.6.2515

The Thermosensitive Lesion in the Replication of the Drug Resistance Factor, Rts1

C G DiJoseph 1, Akira Kaji 1
PMCID: PMC388490  PMID: 4601591

Abstract

The DNA of the thermosensitive R factor, Rts1, has been examined by the technique of sedimentation in alkaline sucrose density gradients. Rts1 DNA was found as closed covalent circles in only a few copies per cell in an Escherichia coli host at the permissive temperature. Rts1 DNA appears to be synthesized at the nonpermissive temperature, but was not found as closed covalent circles. However, circular DNA could be recovered upon shift down to the permissive temperature. The large number of plasmid-negative cells which accumulate after prolonged culture at non-permissive temperature may be due to a strong selective pressure favoring the growth of rare R- segregants.

Keywords: plasmid, circularization of DNA, curing, alkaline sucrose

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clowes R. C. Molecular structure of bacterial plasmids. Bacteriol Rev. 1972 Sep;36(3):361–405. doi: 10.1128/br.36.3.361-405.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cohen S. N., Miller C. A. Multiple molecular species of circular R-factor DNA isolated from Escherichia coli. Nature. 1969 Dec 27;224(5226):1273–1277. doi: 10.1038/2241273a0. [DOI] [PubMed] [Google Scholar]
  3. Cooper S., Helmstetter C. E. Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):519–540. doi: 10.1016/0022-2836(68)90425-7. [DOI] [PubMed] [Google Scholar]
  4. DiJoseph C. G., Bayer M. E., Kaji A. Host cell growth in the presence of the thermosensitive drug resistance factor, Rts1. J Bacteriol. 1973 Jul;115(1):399–410. doi: 10.1128/jb.115.1.399-410.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Falkow S., Tompkins L. S., Silver R. P., Guerry P., Le Blanc D. J. The problems of drug-resistant pathogenic bacteria. The replication of R-factor DNA in Escherichia coli K-12 following conjugation. Ann N Y Acad Sci. 1971 Jun 11;182:153–171. doi: 10.1111/j.1749-6632.1971.tb30654.x. [DOI] [PubMed] [Google Scholar]
  6. Folkmanis A., Freifelder D. Dimers of escherichia coli F' factors. Biochem Biophys Res Commun. 1973 Apr 2;51(3):645–650. doi: 10.1016/0006-291x(73)91363-6. [DOI] [PubMed] [Google Scholar]
  7. Freifelder D., Folkmanis A., Kirschner I. Studies on Escherichia coli sex factors: evidence that covalent circles exist within cells and the general problem of isolation of covalent circles. J Bacteriol. 1971 Mar;105(3):722–727. doi: 10.1128/jb.105.3.722-727.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Freifelder D. Studies with Escherichia coli sex factors. Cold Spring Harb Symp Quant Biol. 1968;33:425–434. doi: 10.1101/sqb.1968.033.01.049. [DOI] [PubMed] [Google Scholar]
  9. GOSS W. A., DEITZ W. H., COOK T. M. MECHANISM OF ACTION OF NALIDIXIC ACID ON ESCHERICHIA COLI.II. INHIBITION OF DEOXYRIBONUCLEIC ACID SYNTHESIS. J Bacteriol. 1965 Apr;89:1068–1074. doi: 10.1128/jb.89.4.1068-1074.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Helinski D. R. Plasmid determined resistance to antibiotics: molecular properties of R factors. Annu Rev Microbiol. 1973;27:437–470. doi: 10.1146/annurev.mi.27.100173.002253. [DOI] [PubMed] [Google Scholar]
  11. Helmstetter C. E., Pierucci O. Cell division during inhibition of deoxyribonucleic acid synthesis in Escherichia coli. J Bacteriol. 1968 May;95(5):1627–1633. doi: 10.1128/jb.95.5.1627-1633.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hudson B., Clayton D. A., Vinograd J. Complex mitochondrial DNA. Cold Spring Harb Symp Quant Biol. 1968;33:435–442. doi: 10.1101/sqb.1968.033.01.050. [DOI] [PubMed] [Google Scholar]
  13. Inselburg J. R factor deoxyribonucleic acid in chromosomeless progeny of Escherichia coli. J Bacteriol. 1971 Feb;105(2):620–628. doi: 10.1128/jb.105.2.620-628.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kontomichalou P., Mitani M., Clowes R. C. Circular R-factor molecules controlling penicillinase synthesis, replicating in Escherichia coli under either relaxed or stringent control. J Bacteriol. 1970 Oct;104(1):34–44. doi: 10.1128/jb.104.1.34-44.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Marunouchi T., Messer W. Deoxyribonucleic acid replication and cell division in Escherichia coli at 33 C. J Bacteriol. 1972 Dec;112(3):1431–1432. doi: 10.1128/jb.112.3.1431-1432.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nisioka T., Mitani M., Clowes R. C. Molecular recombination between R-factor deoxyribonucleic acid molecules in Escherichia coli host cells. J Bacteriol. 1970 Jul;103(1):166–177. doi: 10.1128/jb.103.1.166-177.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nisioka T., Mitani M., Clowes R. Composite circular forms of R factor deoxyribonucleic acid molecules. J Bacteriol. 1969 Jan;97(1):376–385. doi: 10.1128/jb.97.1.376-385.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stadler J., Adelberg E. A. Temperature dependence of sex-factor maintenance in Escherichia coli K-12. J Bacteriol. 1972 Jan;109(1):447–449. doi: 10.1128/jb.109.1.447-449.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stárka J., Moravová J. Cellular division of penicillin-induced filaments of Escherichia coli. Folia Microbiol (Praha) 1967;12(3):240–247. doi: 10.1007/BF02868738. [DOI] [PubMed] [Google Scholar]
  20. Terawaki Y., Kakizawa Y., Takayasu H., Yoshikawa M. Temperature sensitivity of cell growth in Escherichia coli associated with the temperature sensitive R(KM) factor. Nature. 1968 Jul 20;219(5151):284–285. doi: 10.1038/219284a0. [DOI] [PubMed] [Google Scholar]
  21. Terawaki Y., Rownd R. Replication of the R factor Rts1 in Proteus mirabilis. J Bacteriol. 1972 Feb;109(2):492–498. doi: 10.1128/jb.109.2.492-498.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Terawaki Y., Takayasu H., Akiba T. Thermosensitive replication of a kanamycin resistance factor. J Bacteriol. 1967 Sep;94(3):687–690. doi: 10.1128/jb.94.3.687-690.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

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