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. 1974 Jul;71(7):2651–2654. doi: 10.1073/pnas.71.7.2651

Role of ATP in Excision Repair of Ultraviolet Radiation Damage in Escherichia coli

E A Waldstein 1,*, R Sharon 1, R Ben-Ishai 1,†
PMCID: PMC388524  PMID: 4605075

Abstract

The effect of ATP on the first step of excision repair of ultraviolet damage in DNA has been studied using toluene-treated E. coli. During postirradiation incubation, five to six times more single-strand breaks are formed in DNA in the presence of exogenous ATP than in its absence. The ATP-dependent as well as the ATP-independent endonucleolytic activities appear to be catalyzed by the same enzyme since both activities are almost completely absent in uvrA and uvrB mutants. An ATP-dependent endonucleolytic activity has been detected in nonirradiated toluene-treated E. coli. It is concluded that ATP is required in vivo for either the incision step of repair or an enzymatic reaction preceding it.

Keywords: permeable cells, incision step of DNA repair, ATP dependent endonuclease

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOYCE R. P., HOWARD-FLANDERS P. RELEASE OF ULTRAVIOLET LIGHT-INDUCED THYMINE DIMERS FROM DNA IN E. COLI K-12. Proc Natl Acad Sci U S A. 1964 Feb;51:293–300. doi: 10.1073/pnas.51.2.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boyle J. M., Setlow R. B. Correlations between host-cell reactivation, ultraviolet reactivation and pyrimidine dimer excision in the DNA of bacteriophage lambda. J Mol Biol. 1970 Jul 14;51(1):131–144. doi: 10.1016/0022-2836(70)90275-5. [DOI] [PubMed] [Google Scholar]
  3. Carrier W. L., Setlow R. B. Endonuclease from Micrococcus luteus which has activity toward ultraviolet-irradiated deoxyribonucleic acid: purification and properties. J Bacteriol. 1970 Apr;102(1):178–186. doi: 10.1128/jb.102.1.178-186.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Friedberg E. C., King J. J. Dark repair of ultraviolet-irradiated deoxyribonucleic acid by bacteriophage T4: purification and characterization of a dimer-specific phage-induced endonuclease. J Bacteriol. 1971 May;106(2):500–507. doi: 10.1128/jb.106.2.500-507.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goldmark P. J., Linn S. Purification and properties of the recBC DNase of Escherichia coli K-12. J Biol Chem. 1972 Mar 25;247(6):1849–1860. [PubMed] [Google Scholar]
  6. Hadden C. T., Billen D., Corrigan K. Stimulation of non-conservative DNA synthesis by ultraviolet light in freeze-treated Bacillus subtilis. Biochim Biophys Acta. 1973 Nov 14;324(4):461–471. doi: 10.1016/0005-2787(73)90205-0. [DOI] [PubMed] [Google Scholar]
  7. Kaplan J. C., Kushner S. R., Grossman L. Enzymatic repair of DNA. 3. Properties of the UV-endonuclease and UV-exonuclease. Biochemistry. 1971 Aug 31;10(18):3315–3324. doi: 10.1021/bi00794a001. [DOI] [PubMed] [Google Scholar]
  8. Kavenoff R. Characterization of the Bacillus subtilis W23 genome by sedimentation. J Mol Biol. 1972 Dec 30;72(3):801–806. doi: 10.1016/0022-2836(72)90192-1. [DOI] [PubMed] [Google Scholar]
  9. Masker W. E., Hanawalt P. C. Ultraviolet-stimulated DNA synthesis in toluenzied Escherichia coli deficient in DNA polymerase I. Proc Natl Acad Sci U S A. 1973 Jan;70(1):129–133. doi: 10.1073/pnas.70.1.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Modrich P., Lehman I. R., Wang J. C. Enzymatic joining of polynucleotides. XI. Reversal of Escherichia coli deoxyribonucleic acid ligase reaction. J Biol Chem. 1972 Oct 10;247(19):6370–6372. [PubMed] [Google Scholar]
  11. Moses R. E., Richardson C. C. Replication and repair of DNA in cells of Escherichia coli treated with toluene. Proc Natl Acad Sci U S A. 1970 Oct;67(2):674–681. doi: 10.1073/pnas.67.2.674. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Myers D. K., Johnson L. D., Chetty K. G. Effect of relative centrifugal force on the apparent size of DNA from Escherichia coli cells. Can J Biochem. 1973 Apr;51(4):397–406. doi: 10.1139/o73-046. [DOI] [PubMed] [Google Scholar]
  13. Nakayama H., Okubo S., Takagi Y. Repair of ultraviolet-damaged DNA in Micrococcus lysodeikticus. I. An endonuclease specific for ultraviolet-irradiated DNA. Biochim Biophys Acta. 1971 Jan 1;228(1):67–82. doi: 10.1016/0005-2787(71)90547-8. [DOI] [PubMed] [Google Scholar]
  14. ROERSCH A., VAN DER KAM P. C., ADEMA J. DARK REACTIVATION OF ULTRAVIOLET IRRADIATED BACTERIOPHAGE DEOXYRIBONUCLEIC ACID IN VITRO. Biochim Biophys Acta. 1964 Feb 17;80:346–348. [PubMed] [Google Scholar]
  15. Rupp W. D., Howard-Flanders P. Discontinuities in the DNA synthesized in an excision-defective strain of Escherichia coli following ultraviolet irradiation. J Mol Biol. 1968 Jan 28;31(2):291–304. doi: 10.1016/0022-2836(68)90445-2. [DOI] [PubMed] [Google Scholar]
  16. SETLOW R. B., CARRIER W. L. THE DISAPPEARANCE OF THYMINE DIMERS FROM DNA: AN ERROR-CORRECTING MECHANISM. Proc Natl Acad Sci U S A. 1964 Feb;51:226–231. doi: 10.1073/pnas.51.2.226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
  18. Segev N., Miller C., Sharon R., Ben-Ishai R. Excision repair of ultraviolet radiation damage in toluene treated Escherichia coli. Biochem Biophys Res Commun. 1973 Jun 19;52(4):1241–1245. doi: 10.1016/0006-291x(73)90633-5. [DOI] [PubMed] [Google Scholar]
  19. Taketo A., Yasuda S., Sekiguchi M. Initial step of excision repair in Escherichia coli: replacement of defective function of uvr mutants by T4 endonuclease V. J Mol Biol. 1972 Sep 14;70(1):1–14. doi: 10.1016/0022-2836(72)90160-x. [DOI] [PubMed] [Google Scholar]
  20. Val'dshtein E. A., Tomilin N. V. O spetsifichnosti u.-f.-éndonukleazy iz M. lysodeikticus. Deistvie na DNK, obraborannuiu gidroksilaminom. Dokl Akad Nauk SSSR. 1971 Oct;200(6):1459–1462. [PubMed] [Google Scholar]
  21. Worcel A., Burgi E. On the structure of the folded chromosome of Escherichia coli. J Mol Biol. 1972 Nov 14;71(2):127–147. doi: 10.1016/0022-2836(72)90342-7. [DOI] [PubMed] [Google Scholar]
  22. Yasuda S., Sekiguchi M. T4 endonuclease involved in repair of DNA. Proc Natl Acad Sci U S A. 1970 Dec;67(4):1839–1845. doi: 10.1073/pnas.67.4.1839. [DOI] [PMC free article] [PubMed] [Google Scholar]

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