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. 1974 Jul;71(7):2663–2667. doi: 10.1073/pnas.71.7.2663

Hepatitis B Antigen: Antigenic Sites Related to Human Serum Proteins Revealed by Affinity Chromatography

A Robert Neurath 1, Alfred M Prince 1, Arnold Lippin 1
PMCID: PMC388527  PMID: 4136767

Abstract

Hepatitis B antigen-associated particles, isolated from sera of antigen carriers, were submitted to affinity chromatography on columns of insolubilized antibodies to normal human plasma. The particles adsorbed to the immunosorbent at pH 7.2 and were subsequently eluted at pH 2.2. Exposure of the particles to 8 M urea, 5 M KI, pH 2.2, detergents, organic solvents, or proteolytic enzymes failed to prevent their subsequent adsorption to the immunosorbent. This suggests that antigenic determinants related to human plasma proteins are constituent components of hepatitis B antigen-associated particles. These determinants are distinct from the group-specific (a) and subtype-specific (d or y) sites of the hepatitis B antigen and appear to be related to antigenic specificities on prealbumin, albumin, apolipoproteins C and D, and the γ-chain of immunoglobulin G.

Keywords: albumin, prealbumin, apolipoproteins, immunoglobulins

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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