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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1974 Aug;71(8):2942–2945. doi: 10.1073/pnas.71.8.2942

Control of the Initiation of DNA Synthesis in 3T3 Cells: Low-Molecular-Weight Nutrients

Robert W Holley 1, Josephine A Kiernan 1
PMCID: PMC388594  PMID: 4528490

Abstract

Sparse 3T3 cells, in excess serum, become quiescent in the early G1 (or G0) phase of the cell cycle if the cells are cultured in low concentrations of amino acids, glucose, or phosphate ion. These quiescent cells then initiate DNA synthesis if the concentration of the limiting nutrient is increased. In normal medium, DNA synthesis in the same 3T3 cell line is controlled by serum factors. The results demonstrate the complexity of the control by external agents of DNA synthesis in these “normal,” density-dependent cells.

Keywords: amino acids, glucose, phosphate, cell cycle

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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