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. 1971 Mar;68(3):641–644. doi: 10.1073/pnas.68.3.641

Specific Transformylation of One Methionyl-tRNA from Cotton Seeding Chloroplasts by Endogenous and Escherichia coli Transformylases

William C Merrick 1, L S Dure III 1,*
PMCID: PMC389007  PMID: 5276774

Abstract

Two isoaccepting chloroplastic and one cytoplasmic tRNAMet species have been separated from germinating cotton cotyledons. The methionylated form of one of the chloroplastic species (but none of the other or of the cytoplasmic tRNAMet) can be formylated either by an endogenous transformylase or by Escherichia coli transformylase.

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Selected References

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  1. Bhaduri S., Chatterjee N. K., Bose K. K., Gupta N. K. Initiation of protein synthesis in rabbit reticulocytes. Biochem Biophys Res Commun. 1970 Jul 27;40(2):402–407. doi: 10.1016/0006-291x(70)91023-5. [DOI] [PubMed] [Google Scholar]
  2. Brown J. C., Smith A. E. Initiator codons in eukaryotes. Nature. 1970 May 16;226(5246):610–612. doi: 10.1038/226610a0. [DOI] [PubMed] [Google Scholar]
  3. Burkard G., Eclancher B., Weil J. H. Presence of N-formyl-methionyl-transfer RNA in bean chloroplasts. FEBS Lett. 1969 Aug;4(4):285–287. doi: 10.1016/0014-5793(69)80257-7. [DOI] [PubMed] [Google Scholar]
  4. Epler J. L., Shugart L. R., Barnett W. E. N-formylmethionyl transfer ribonucleic acid in mitochondria from Neurospora. Biochemistry. 1970 Sep 1;9(18):3575–3579. doi: 10.1021/bi00820a011. [DOI] [PubMed] [Google Scholar]
  5. Gallo R. C., Pestka S. Transfer RNA species in normal and leukemic human lymphoblasts. J Mol Biol. 1970 Sep 14;52(2):195–219. doi: 10.1016/0022-2836(70)90025-2. [DOI] [PubMed] [Google Scholar]
  6. Gillam I., Blew D., Warrington R. C., von Tigerstrom M., Tener G. M. A general procedure for the isolation of specific transfer ribonucleic acids. Biochemistry. 1968 Oct;7(10):3459–3468. doi: 10.1021/bi00850a022. [DOI] [PubMed] [Google Scholar]
  7. Housman D., Jacobs-Lorena M., Rajbhandary U. L., Lodish H. F. Initiation of haemoglobin synthesis by methionyl-tRNA. Nature. 1970 Aug 29;227(5261):913–918. doi: 10.1038/227913a0. [DOI] [PubMed] [Google Scholar]
  8. Jackson R., Hunter T. Role of methionine in the initiation of haemoglobin synthesis. Nature. 1970 Aug 15;227(5259):672–676. doi: 10.1038/227672a0. [DOI] [PubMed] [Google Scholar]
  9. Kelmers A. D., Novelli G. D., Stulberg M. P. Separation of transfer ribonucleic acids by reverse phase chromatography. J Biol Chem. 1965 Oct;240(10):3979–3983. [PubMed] [Google Scholar]
  10. Leis J. P., Keller E. B. Protein Chain-Initiating Methionine tRNAs in Chloroplasts and Cytoplasm of Wheat Leaves. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1593–1599. doi: 10.1073/pnas.67.3.1593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Leis J. P., Keller E. B. Protein chain initiation by methionyl-tRNA. Biochem Biophys Res Commun. 1970 Jul 27;40(2):416–421. doi: 10.1016/0006-291x(70)91025-9. [DOI] [PubMed] [Google Scholar]
  12. Ljungdahl L., Brewer J. M., Neece S. H., Fairwell T. Purification, stability, and composition of formyltetrahydrofolate synthetase from Clostridium thermoaceticum. J Biol Chem. 1970 Sep 25;245(18):4791–4797. [PubMed] [Google Scholar]
  13. Loening U. E. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem J. 1967 Jan;102(1):251–257. doi: 10.1042/bj1020251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schwartz J. H., Meyer R., Eisenstadt J. M., Brawerman G. Involvement of N-formylmethionine in initiation of protein synthesis in cell-free extracts of Euglena gracilis. J Mol Biol. 1967 May 14;25(3):571–574. doi: 10.1016/0022-2836(67)90210-0. [DOI] [PubMed] [Google Scholar]
  15. Shafritz D. A., Anderson W. F. Factor dependent binding of methionyl-tRNAs to reticulocyte ribosomes. Nature. 1970 Aug 29;227(5261):918–920. doi: 10.1038/227918a0. [DOI] [PubMed] [Google Scholar]
  16. Smith A. E., Marcker K. A. Cytoplasmic methionine transfer RNAs from eukaryotes. Nature. 1970 May 16;226(5246):607–610. doi: 10.1038/226607a0. [DOI] [PubMed] [Google Scholar]
  17. Stocking C. R. Chloroplast Isolation in Nonaqueous Media. Plant Physiol. 1959 Jan;34(1):56–61. doi: 10.1104/pp.34.1.56. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Weiss J. F., Kelmers A. D. A new chromatographic system for increased resolution of transfer ribonucleic acids. Biochemistry. 1967 Aug;6(8):2507–2513. doi: 10.1021/bi00860a030. [DOI] [PubMed] [Google Scholar]
  19. Wigle D. T., Dixon G. H. Transient incorporation of methionine at the N-terminus of protamine newly synthesized in trout testis cells. Nature. 1970 Aug 15;227(5259):676–680. doi: 10.1038/227676a0. [DOI] [PubMed] [Google Scholar]
  20. Williams G. R., Williams A. S. Hybridization of bean leaf leucyl-tRNA with nuclear DNA and with chloroplast DNA. Biochem Biophys Res Commun. 1970 Jun 5;39(5):858–863. doi: 10.1016/0006-291x(70)90402-x. [DOI] [PubMed] [Google Scholar]
  21. Wilson D. B., Dintzis H. M. Protein chain initiation in rabbit reticulocytes. Proc Natl Acad Sci U S A. 1970 Aug;66(4):1282–1289. doi: 10.1073/pnas.66.4.1282. [DOI] [PMC free article] [PubMed] [Google Scholar]

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