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. 1971 Apr;68(4):795–798. doi: 10.1073/pnas.68.4.795

Isolation of Transducing Particles of ϕ80 Bacteriophage That Carry Different Regions of the Escherichia coli Genome

R Press 1, N Glansdorff 1,*, P Miner 1, J De Vries 1, R Kadner 1,†, W K Maas 1
PMCID: PMC389045  PMID: 4927673

Abstract

It has been possible to mate two strains harboring F-prime (F′) factors and to isolate from such matings rare recombinants that behave as though the two episomes had fused. Thus, two genes not previously linked may be brought into close proximity.

An F′ factor carrying the attachment site for ϕ80 was fused with one carrying the met-ppc-arg region of the chromosome. Lysogenization of such a strain, followed by induction, led to the isolation of ϕ80arg+ and ϕ80met+ transducing phages. This technique may be utilized as a general method for joining diverse bacterial genes to the genome of phage ϕ80.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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