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. 1971 Apr;68(4):795–798. doi: 10.1073/pnas.68.4.795

Isolation of Transducing Particles of ϕ80 Bacteriophage That Carry Different Regions of the Escherichia coli Genome

R Press 1, N Glansdorff 1,*, P Miner 1, J De Vries 1, R Kadner 1,†, W K Maas 1
PMCID: PMC389045  PMID: 4927673

Abstract

It has been possible to mate two strains harboring F-prime (F′) factors and to isolate from such matings rare recombinants that behave as though the two episomes had fused. Thus, two genes not previously linked may be brought into close proximity.

An F′ factor carrying the attachment site for ϕ80 was fused with one carrying the met-ppc-arg region of the chromosome. Lysogenization of such a strain, followed by induction, led to the isolation of ϕ80arg+ and ϕ80met+ transducing phages. This technique may be utilized as a general method for joining diverse bacterial genes to the genome of phage ϕ80.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beckwith J. R., Signer E. R. Transposition of the lac region of Escherichia coli. I. Inversion of the lac operon and transduction of lac by phi80. J Mol Biol. 1966 Aug;19(2):254–265. doi: 10.1016/s0022-2836(66)80003-7. [DOI] [PubMed] [Google Scholar]
  2. CLARK A. J., MARGULIES A. D. ISOLATION AND CHARACTERIZATION OF RECOMBINATION-DEFICIENT MUTANTS OF ESCHERICHIA COLI K12. Proc Natl Acad Sci U S A. 1965 Feb;53:451–459. doi: 10.1073/pnas.53.2.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CUZIN F. [Autonomous multiplication of the sexual episome of Escherichia coli K12 n an Hfr strain]. C R Hebd Seances Acad Sci. 1962 Jun 13;254:4211–4213. [PubMed] [Google Scholar]
  4. Cunin R., Elseviers D., Sand G., Freundlich G., Glandsdorff N. On the functional organization of the arg ECBH cluster of genes in Escherichia coli K-12. Mol Gen Genet. 1969;106(1):32–47. doi: 10.1007/BF00332819. [DOI] [PubMed] [Google Scholar]
  5. Cuzin F., Jacob F. Association stable de deux épisomes F différents dans un clone d'Escherichia coli. Ann Inst Pasteur (Paris) 1967 Aug;113(2):145–155. [PubMed] [Google Scholar]
  6. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966 Jul;54(1):61–76. doi: 10.1093/genetics/54.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. GLANSDORFF N. TOPOGRAPHY OF COTRANSDUCIBLE ARGININE MUTATIONS IN ESCHERICHIA COLI K-12. Genetics. 1965 Feb;51:167–179. doi: 10.1093/genetics/51.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gottesman S., Beckwith J. R. Directed transposition of the arabinose operon: a technique for the isolation of specialized transducing bacteriophages for any Escherichia coli gene. J Mol Biol. 1969 Aug 28;44(1):117–127. doi: 10.1016/0022-2836(69)90408-2. [DOI] [PubMed] [Google Scholar]
  10. HAYES W. The kinetics of the mating process in Escherichia coli. J Gen Microbiol. 1957 Feb;16(1):97–119. doi: 10.1099/00221287-16-1-97. [DOI] [PubMed] [Google Scholar]
  11. Hercules K. D., Knacht R., Zubay G. Differential bacteriophage DNA replication after induction of a strain of Escherichia coli doubly lysogenic for phi80 and phi80dlac. Virology. 1968 Feb;34(2):337–343. doi: 10.1016/0042-6822(68)90244-4. [DOI] [PubMed] [Google Scholar]
  12. Low B. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc Natl Acad Sci U S A. 1968 May;60(1):160–167. doi: 10.1073/pnas.60.1.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MAAS R. EXCLUSION OF A FLAC EPISOME BY AN HFR GENE. Proc Natl Acad Sci U S A. 1963 Dec;50:1051–1055. doi: 10.1073/pnas.50.6.1051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Maas W. K., Goldschmidt A. D. A mutant of Escherichia coli permitting replication of two F factors. Proc Natl Acad Sci U S A. 1969 Mar;62(3):873–880. doi: 10.1073/pnas.62.3.873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Signer E. R. Interaction of prophages at the att80 site with the chromosome of Escherichia coli. J Mol Biol. 1966 Jan;15(1):243–255. doi: 10.1016/s0022-2836(66)80224-3. [DOI] [PubMed] [Google Scholar]
  16. Taylor A. L., Trotter C. D. Revised linkage map of Escherichia coli. Bacteriol Rev. 1967 Dec;31(4):332–353. doi: 10.1128/br.31.4.332-353.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

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