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. 1971 May;68(5):1014–1018. doi: 10.1073/pnas.68.5.1014

Partial Purification by Affinity Chromatography of Tyrosine Aminotransferase-Synthesizing Ribosomes from Hepatoma Tissue Culture Cells

James V Miller Jr *, Pedro Cuatrecasas , E Brad Thompson *
PMCID: PMC389103  PMID: 4396918

Abstract

This report describes a cell-free protein-synthesizing system derived from hepatoma tissue culture cells in which the synthesis of tyrosine aminotransferase is enhanced in relation to total protein synthesis. The enhancement was achieved by using affinity chromatography to select a specific fraction of ribosomes in which the proportion of tyrosine aminotransferase synthesis is higher than it is in unfractionated ribosomes.

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Selected References

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