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. 1971 Jun;68(6):1174–1177. doi: 10.1073/pnas.68.6.1174

Transduction of the Nitrogen-Fixation Genes in Klebsiella pneumoniae

Stanley Streicher 1, Elizabeth Gurney 1, Raymond C Valentine 1
PMCID: PMC389145  PMID: 5288365

Abstract

The bacteriophage P1 infects and functions as a generalized transducing phage for nitrogen-fixing strains of the coliform bacterium Klebsiella pneumoniae. Bacterial mutants (nif-) unable to grow on molecular nitrogen as a nitrogen source were found to be deficient in nitrogenase activity as assayed by the conversion of acetylene to ethylene. These mutants regained normal nitrogenase activity and the ability to grow on N2 after transduction with lysates of P1 phage prepared from wild-type bacteria. Transductional analysis with P1 revealed that several nif genes are located on the genetic linkage map of Klebsiella near the histidine operon.

Keywords: mutants, P1 phage, histidine operon, nitrogenase

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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