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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1971 Jun;68(6):1326–1330. doi: 10.1073/pnas.68.6.1326

Effect of Double-Stranded Viral RNA on Mammalian Cells in Culture

B Cordell-Stewart 1, Milton W Taylor 1
PMCID: PMC389182  PMID: 4331088

Abstract

During bovine enterovirus infection of Ehrlich ascites tumor cells, large amounts of double-stranded RNA accumulate. Addition of this double-stranded RNA to uninfected cells leads to rapid cell death. This is not a result of infectious virus production. Neither single-stranded RNA nor heat-denatured double-stranded RNA has this effect. Similar experiments with synthetic double-stranded polymers, poly(I)·poly(C) and poly(A)·poly(U), show that they are only slightly toxic at the concentrations used. The effect of the double-stranded RNA is nonspecific for cells of different origins. The implications of this finding in relation to the cytopathic effects of picornavirus and to cancer chemotherapy are discussed.

Keywords: bovine enterovirus, picornavirus, interferon, tumor regression

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amako K., Dales S. Cytopathology of Mengovirus infection. I. Relationship between cellular disintegration and virulence. Virology. 1967 Jun;32(2):184–200. doi: 10.1016/0042-6822(67)90269-3. [DOI] [PubMed] [Google Scholar]
  2. Amako K., Dales S. Cytopathology of Mengovirus infection. II. Proliferation of membranous cisternae. Virology. 1967 Jun;32(2):201–215. doi: 10.1016/0042-6822(67)90270-x. [DOI] [PubMed] [Google Scholar]
  3. Arlinghaus R. B., Polatnick J., Vande Woude G. F. Studies on foot-and-mouth disease virus ribonucleic acid synthesis. Virology. 1966 Nov;30(3):541–550. doi: 10.1016/0042-6822(66)90129-2. [DOI] [PubMed] [Google Scholar]
  4. Baltimore D., Girard M., Darnell J. E. Aspects of the synthesis of poliovirus RNA and the formation of virus particles. Virology. 1966 Jun;29(2):179–189. doi: 10.1016/0042-6822(66)90024-9. [DOI] [PubMed] [Google Scholar]
  5. Bishop J. M., Koch G. Purification and characterization of poliovirus-induced infectious double-stranded ribonucleic acid. J Biol Chem. 1967 Apr 25;242(8):1736–1743. [PubMed] [Google Scholar]
  6. Carter W. A., Levy H. B. Ribosomes: effect of interferon on their interaction with rapidly labeled cellular and viral RNA's. Science. 1967 Mar 10;155(3767):1254–1257. doi: 10.1126/science.155.3767.1254. [DOI] [PubMed] [Google Scholar]
  7. Ehrenfeld E., Hunt T. Double-stranded poliovirus RNA inhibits initiation of protein synthesis by reticulocyte lysates. Proc Natl Acad Sci U S A. 1971 May;68(5):1075–1078. doi: 10.1073/pnas.68.5.1075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HOLLAND J. J. INHIBITION OF HOST CELL MACROMOLECULAR SYNTHESIS BY HIGH MULTIPLICITIES OF POLIOVIRUS UNDER CONDITIONS PREVENTING VIRUS SYNTHESIS. J Mol Biol. 1964 Apr;8:574–581. doi: 10.1016/s0022-2836(64)80012-7. [DOI] [PubMed] [Google Scholar]
  9. Haase A. T., Levy H., Baron S., Kasel J. A. Mengovirus-induced cytopathic effect in L-cells: protective effect of interferon. J Virol. 1969 Oct;4(4):490–495. doi: 10.1128/jvi.4.4.490-495.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Koch G., Quintrell N., Bishop J. M. An agar cell-suspension plaque assay for isolated viral RNA. Biochem Biophys Res Commun. 1966 Aug 12;24(3):304–309. doi: 10.1016/0006-291x(66)90155-0. [DOI] [PubMed] [Google Scholar]
  11. LWOFF A., DULBECCO R., VOGT M., LWOFF M. Kinetics of the release of poliomyelitis virus from single cells. Virology. 1955 May;1(1):128–139. doi: 10.1016/0042-6822(55)90010-6. [DOI] [PubMed] [Google Scholar]
  12. Larson V. M., Clark W. R., Hilleman M. R. Influence of synthetic (poly I:C) and viral double-stranded ribonucleic acids on adenovirus 12 oncogenesis in hamsters. Proc Soc Exp Biol Med. 1969 Jul;131(3):1002–1011. doi: 10.3181/00379727-131-34028. [DOI] [PubMed] [Google Scholar]
  13. Levy H. B., Law L. W., Rabson A. S. Inhibition of tumor growth by polyinosinic-polycytidylic acid. Proc Natl Acad Sci U S A. 1969 Feb;62(2):357–361. doi: 10.1073/pnas.62.2.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Levy H. B., Riley F. The effect of polyinosinic:polycytidylic acid on tumor metabolism. Proc Soc Exp Biol Med. 1970 Oct;135(1):141–145. doi: 10.3181/00379727-135-35006. [DOI] [PubMed] [Google Scholar]
  15. Marcus P. I., Salb J. M. Molecular basis of interferon action: inhibition of viral RNA translation. Virology. 1966 Nov;30(3):502–516. doi: 10.1016/0042-6822(66)90126-7. [DOI] [PubMed] [Google Scholar]
  16. Ralph R. K. Double-stranded viral RNA. Adv Virus Res. 1969;15:61–158. doi: 10.1016/s0065-3527(08)60874-x. [DOI] [PubMed] [Google Scholar]
  17. Taylor M. W., Cordell B., Souhrada M., Prather S. Viruses as an aid to cancer therapy: regression of solid and ascites tumors in rodents after treatment with bovine enterovirus. Proc Natl Acad Sci U S A. 1971 Apr;68(4):836–840. doi: 10.1073/pnas.68.4.836. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Taylor M. W., Davidson J. N., Land C., Wall R. Virus-induced regression of tumor growth. J Natl Cancer Inst. 1970 Mar;44(3):515–519. [PubMed] [Google Scholar]
  19. Wall R., Taylor M. W. Host-dependent restriction of mengovirus replication. J Virol. 1969 Nov;4(5):681–687. doi: 10.1128/jvi.4.5.681-687.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wall R., Taylor M. W. Mengovirus RNA synthesis in productive and restrictive cell lines. Virology. 1970 Sep;42(1):78–86. doi: 10.1016/0042-6822(70)90240-0. [DOI] [PubMed] [Google Scholar]
  21. YOON C. H. INCORPORATION OF EXOGENOUS RNA INTO NELSON'S ASCITES CELLS IN VITRO. Exp Cell Res. 1965 May;38:386–397. doi: 10.1016/0014-4827(65)90412-x. [DOI] [PubMed] [Google Scholar]

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