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. 1971 Jun;68(6):1351–1354. doi: 10.1073/pnas.68.6.1351

An Alternate Complement Pathway: C-3 Cleaving Activity, Not Due to [unk], on Endotoxic Lipopolysaccharide after Treatment with Guinea Pig Serum; Relation to Properdin

Robert L Marcus 1,2, Hyun S Shin 1,2, Manfred M Mayer 1,2
PMCID: PMC389187  PMID: 5288385

Abstract

The reaction between endotoxic lipopolysaccharide (LPS) and the guinea pig complement system was shown to proceed by way of an intermediate complex, LPS-X, which contains at least six guinea pig serum proteins. LPS-X, like [unk] (sheep erythrocytes carrying antibody molecules and [unk] complexes), destroys the C3 molecule by cleavage. On incubation at 37°C, LPS-X loses its capacity to destroy C3 at about the same rate as the decay of [unk], so that it has been assumed that LPS-X carries [unk] sites that are responsible for the destruction of C3.

We have now shown that monospecific rabbit antiguinea pig C2, which effectively inhibits C3 cleavage by [unk], does not interfere with the destruction of C3 by LPS-X. Furthermore, not more than a trace of C2ad is released from LPS-X on incubation at 37°C. These results indicate that LPS-X does not carry a significant quantity of [unk] and, hence, that its capacity to destroy C3 is due to another factor which is presumably a component of the properdin system.

Keywords: complement components

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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