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. 1971 Jul;68(7):1450–1455. doi: 10.1073/pnas.68.7.1450

Antibodies Reactive with Native Lysozyme Elicited by a Completely Synthetic Antigen

Ruth Arnon 1, Elchanan Maron 1, Michael Sela 1, Christian B Anfinsen 1,*
PMCID: PMC389215  PMID: 5283934

Abstract

A synthetic peptide consisting of the aminoacid sequence of residues 64-82 of lysozyme, with alanine replacing cysteine as residue 76, was prepared by the solid-phase technique. Mild reduction followed by reoxidation in air of the deprotected peptide led to the formation of a closed loop containing an intrachain disulfide bond. A conjugate consisting of this “loop” attached to multi-poly(DL-alanyl)-poly(L-lysine) elicited, in rabbits and goats, the formation of antibodies capable of reacting with lysozyme and with the loop peptide prepared from it. These immunological interactions can be inhibited by either lysozyme or the loop peptide, but not by the performic acid-oxidized open-chain peptide. Thus, the antibodies elicited by the completely synthetic antigen show specificity toward the “loop” structure (residues 64-80) of native lysozyme.

Keywords: lysozyme residues 64-82, loop

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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