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. 1971 Jul;68(7):1512–1515. doi: 10.1073/pnas.68.7.1512

Acetyl CoA Carboxylase: Isolation and Characterization of Native Biotin Carboxyl Carrier Protein

R Ray Fall 1, A M Nervi 1,*, Alfred W Alberts 1, P Roy Vagelos 1
PMCID: PMC389229  PMID: 4934522

Abstract

A large form of biotin carboxyl carrier protein (BCCPL) has been isolated from extracts of Escherichia coli. It has a minimal molecular weight of 20,000, according to its behavior on sodium dodecylsulfate-polyacrylamide gel electrophoresis, and contains approximately 1 mol of biotin per 22,000 g of protein. BCCPL exhibits Km values, in the biotin carboxylase and transcarboxylase half-reactions of acetyl CoA carboxylase, of 2 × 10-7 M and 4 × 10-7 M, respectively; these values are 50-100 times lower than those obtained with smaller forms of BCCP previously isolated. Electrophoresis of crude extracts of E. coli indicates that the major biotin-containing protein migrates at the same rate as BCCPL, which suggests that BCCPL is the native form of BCCP in E. coli.

Keywords: high molecular weight, high activity

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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