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. 1971 Jul;68(7):1520–1524. doi: 10.1073/pnas.68.7.1520

Transformation of Mouse 3T3 Cells by Murine Sarcoma Virus: Release of Virus-Like Particles in the Absence of Replicating Murine Leukemia Helper Virus

R H Bassin 1, L A Phillips 1, M J Kramer 1,*, D K Haapala 1, P T Peebles 1, Shigeko Nomura 1, P J Fischinger 1
PMCID: PMC389231  PMID: 4327005

Abstract

Small numbers of virus-like particles were observed by electron microscopy in each of two cloned lines of 3T3 cells transformed by murine sarcoma virus, even though these lines were free of detectable quantities of infectious leukemia and sarcoma virus. The morphology and occurrence of the particles were identical to those of the murine leukemia-sarcoma group. Moreover, the particles incorporated uridine and had a buoyant density of 1.16 g/ml in sucrose gradients. No evidence of sarcoma or leukemia virus infectivity was associated with the particles in cells of several susceptible species under various conditions, including both cosedimentation with leukemia virus and infection in the presence of inactivated Sendai virus. The particles may represent a form of murine sarcoma virus deficient in one or more of the viral components necessary for infectivity.

Keywords: density gradient centrifugation, electron microscopy, [3H]uridine

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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