Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1971 Jul;68(7):1598–1602. doi: 10.1073/pnas.68.7.1598

Selective Induction by Nerve Growth Factor of Tyrosine Hydroxylase and Dopamine-β-Hydroxylase in the Rat Superior Cervical Ganglia

H Thoenen *, P U Angeletti †, R Levi-Montalcini †, R Kettler *
PMCID: PMC389249  PMID: 5283951

Abstract

Treatment of newborn rats with 10 μg/g of nerve growth factor for 10 days enhanced not only the growth but also the differentiation of neuroblasts in superior cervical ganglia. These morphological changes were accompanied by selective induction of tyrosine hydroxylase and dopamine β-hydroxylase (EC 1.14.2.1), whereas the total and specific activities of other enzymes involved in biosynthesis or metabolic degradation of norepinephrine rose only in proportion to the increase in volume of the sympathetic ganglia. There are remarkable similarities between this effect of nerve growth factor and the induction of trans-synaptic enzymes by increased activity of the sympathetic nervous system.

Keywords: dopa decarboxylase, monoamine oxidase

Full text

PDF
1600

Images in this article

1599Image
on p.1599
1600Image
on p.1600

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bocchini V., Angeletti P. U. The nerve growth factor: purification as a 30,000-molecular-weight protein. Proc Natl Acad Sci U S A. 1969 Oct;64(2):787–794. doi: 10.1073/pnas.64.2.787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CLELAND W. W. Computer programmes for processing enzyme kinetic data. Nature. 1963 May 4;198:463–465. doi: 10.1038/198463a0. [DOI] [PubMed] [Google Scholar]
  3. Håkanson R., Owman C. Pineal dopa decarboxylase and monoamine oxidase activities as related to the monoamine stores. J Neurochem. 1966 Jul;13(7):597–605. doi: 10.1111/j.1471-4159.1966.tb11956.x. [DOI] [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Levi-Montalcini R., Angeletti P. U. Nerve growth factor. Physiol Rev. 1968 Jul;48(3):534–569. doi: 10.1152/physrev.1968.48.3.534. [DOI] [PubMed] [Google Scholar]
  6. Levi-Montalcini R., Caramia F., Luse S. A., Angeletti P. U. In vitro effects of the nerve growth factor on the fine structure of the sensory nerve cells. Brain Res. 1968 May;8(2):347–362. doi: 10.1016/0006-8993(68)90054-1. [DOI] [PubMed] [Google Scholar]
  7. Levi-Montalcini R. The nerve growth factor: its mode of action on sensory and sympathetic nerve cells. Harvey Lect. 1966;60:217–259. [PubMed] [Google Scholar]
  8. Levitt M., Gibb J. W., Daly J. W., Lipton M., Udenfriend S. A new class of tyrosine hydroxylase inhibitors and a simple assay of inhibition in vivo. Biochem Pharmacol. 1967 Jul 7;16(7):1313–1321. doi: 10.1016/0006-2952(67)90162-1. [DOI] [PubMed] [Google Scholar]
  9. Molinoff P. B., Brimijoin S., Weinshilboum R., Axelrod J. Neurally mediated increase in dopamine-beta-hydroxylase activity. Proc Natl Acad Sci U S A. 1970 Jun;66(2):453–458. doi: 10.1073/pnas.66.2.453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mueller R. A., Thoenen H., Axelrod J. Inhibition of trans-synaptically increased tyrosine hydroxylase activity by cycloheximide and actinomycin D. Mol Pharmacol. 1969 Sep;5(5):463–469. [PubMed] [Google Scholar]
  11. WILKINSON G. N. Statistical estimations in enzyme kinetics. Biochem J. 1961 Aug;80:324–332. doi: 10.1042/bj0800324. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES