Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1971 Jul;68(7):1631–1633. doi: 10.1073/pnas.68.7.1631

Hormone-Controlled Synthesis of Endoplasmic Reticulum in Barley Aleurone Cells

Warren H Evins 1,*, J E Varner 1
PMCID: PMC389256  PMID: 5283956

Abstract

The rate of synthesis of the endoplasmic reticulum in barley aleurone cells after treatment with gibberellic acid was determined by measurement of [14C]-choline incorporation into acid-insoluble material in a semipurified fraction containing the endoplasmic reticulum. 94% of the 14C incorporated into this fraction is extractable by lipid solvents and only 9% is removed by procedures for nucleic acid extraction. Gibberellic acid increases the rate of synthesis of the endoplasmic reticulum 4- to 8-fold, starting about 4 hr after addition of the hormone (at about the same time as polysome formation). Abscisic acid inhibits this gibberellic acid-enhanced increase in the rate of synthesis of endoplasmic reticulum.

Keywords: gibberellic acid, abscisic acid

Full text

PDF
1632

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BIEBER L. L., HODGSON E., CHELDELIN V. H., BROOKES V. J., NEWBURGH R. W. Phospholipid patterns in the blowfly, Phormia regina (Meigen). J Biol Chem. 1961 Oct;236:2590–2595. [PubMed] [Google Scholar]
  2. Bieber L. L., Sellers L. G., Kumar S. S. Studies on the formation and degradation of phosphatidyl-beta-methylcholine, beta-methylcholine derivatives, and carnitine by housefly larvae. J Biol Chem. 1969 Feb 25;244(4):630–636. [PubMed] [Google Scholar]
  3. Campbell P. N. Functions of polyribosomes attached to membranes of animal cells. FEBS Lett. 1970 Mar 16;7(1):1–7. doi: 10.1016/0014-5793(70)80603-2. [DOI] [PubMed] [Google Scholar]
  4. Chrispeels M. J., Varner J. E. Gibberellic Acid-enhanced synthesis and release of alpha-amylase and ribonuclease by isolated barley and aleurone layers. Plant Physiol. 1967 Mar;42(3):398–406. doi: 10.1104/pp.42.3.398. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chrispeels M. J., Varner J. E. Hormonal control of enzyme synthesis: on the mode of action of gibberellic Acid and abscisin in aleurone layers of barley. Plant Physiol. 1967 Jul;42(7):1008–1016. doi: 10.1104/pp.42.7.1008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Filner P., Varner J. E. A test for de novo synthesis of enzymes: density labeling with H2O18 of barley alpha-amylase induced by gibberellic acid. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1520–1526. doi: 10.1073/pnas.58.4.1520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hallinan T., Duffy T., Waddington S., Munro H. N. Incorporation of 14-C-choline into phospholipids of rat liver microsome subfractions: evidence against formation of endoplasmic reticulum from the nuclear membrane. Q J Exp Physiol Cogn Med Sci. 1966 Apr;51(2):142–150. doi: 10.1113/expphysiol.1966.sp001835. [DOI] [PubMed] [Google Scholar]
  8. Jacobsen J. V., Varner J. E. Gibberellic Acid-induced synthesis of protease by isolated aleurone layers of barley. Plant Physiol. 1967 Nov;42(11):1596–1600. doi: 10.1104/pp.42.11.1596. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Khan A. A., Anojulu C. C. Abscisic acid induced changes in nucleotide composition of rapidly labelled RNA species of pear embryos. Biochem Biophys Res Commun. 1970 Mar 27;38(6):1069–1075. doi: 10.1016/0006-291x(70)90348-7. [DOI] [PubMed] [Google Scholar]
  10. Khan A. A., Heit C. E. Selective effect of hormones on nucleic acid metabolism during germination of pear embryos. Biochem J. 1969 Jul;113(4):707–712. doi: 10.1042/bj1130707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Nagley P., Hallinan T. The use of radioactive choline as a label for microsomal membranes. I. Selectivity of label for endoplasmic reticulum and specificity for lecithin. Biochim Biophys Acta. 1968 Sep 17;163(2):218–225. doi: 10.1016/0005-2736(68)90100-4. [DOI] [PubMed] [Google Scholar]
  13. SIEKEVITZ P., PALADE G. E. A cytochemical study on the pancreas of the guinea pig. 5. In vivo incorporation of leucine-1-C14 into the chymotrypsinogen of various cell fractions. J Biophys Biochem Cytol. 1960 Jul;7:619–630. doi: 10.1083/jcb.7.4.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. STAEHELIN T., WETTSTEIN F. O., OURA H., NOLL H. DETERMINATION OF THE CODING RATIO BASED ON MOLECULAR WEIGHT OF MESSENGER RIBONUCLEIC ACID ASSOCIATED WITH ERGOSOMES OF DIFFERENT AGGREGATE SIZE. Nature. 1964 Jan 18;201:264–270. doi: 10.1038/201264a0. [DOI] [PubMed] [Google Scholar]
  15. Tata J. R. Hormonal regulation of growth and protein synthesis. Nature. 1968 Jul 27;219(5152):331–337. doi: 10.1038/219331a0. [DOI] [PubMed] [Google Scholar]
  16. WETTSTEIN F. O., STAEHELIN T., NOLL H. Ribosomal aggregate engaged in protein synthesis: characterization of the ergosome. Nature. 1963 Feb 2;197:430–435. doi: 10.1038/197430a0. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES