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. 1971 Aug;68(8):1858–1861. doi: 10.1073/pnas.68.8.1858

Biochemical Differentiation in Reaggregating Brain Cell Culture

Nicholas W Seeds 1,2
PMCID: PMC389308  PMID: 4108524

Abstract

Dissociated cells from embryonic mouse brain reassociate in rotation culture to form aggregates. During cell culture the specific activities of choline acetyl-transferase (EC 2.3.1.6), acetylcholinesterase (EC 3.1.1.7), and glutamate decarboxylase (EC 4.1.1.15) in the aggregates increase up to twenty-fold, a phenomenon that approximates some of the biochemical events in the development of the mouse brain.

Keywords: morphology, enzyme assays, mouse embryo

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Selected References

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