Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1971 Sep;68(9):2198–2202. doi: 10.1073/pnas.68.9.2198

Inhibition of the DNA Polymerase of Rauscher Leukemia Virus by Single-Stranded Polyribonucleotides

F William Tuominen 1,*, Francis T Kenney 1
PMCID: PMC389384  PMID: 5289379

Abstract

The DNA polymerase of Rauscher murine leukemia virus is strongly and specifically inhibited by nontemplate, single-stranded polyribonucleotides with either the resident viral RNA, native calf-thymus DNA, or poly[d(A-T)] as templates. These inhibitory homopolymers are apparently bound to the template site of the polymerase, since they interact competitively with the template. The strength of the inhibition depends on the particular homopolymer used: poly(U) > poly(G) » poly(A) > poly(C). The Ki for poly(U) was 0.08 μg/ml, which represents an apparent affinity six times greater than that observed for viral RNA. No such inhibition was observed with a highly purified DNA polymerase from mouse embryos or the Escherichia coli enzyme.

Keywords: mouse, poly(U)

Full text

PDF
2199

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ackermann W. W., Murphy W. H., Miller B. A., Kurtz H., Barker S. T. RNA dependent DNA synthesis in cell free preparations of human leukemia cells. Biochem Biophys Res Commun. 1971 Feb 19;42(4):723–729. doi: 10.1016/0006-291x(71)90547-x. [DOI] [PubMed] [Google Scholar]
  2. Alberts B. M., Amodio F. J., Jenkins M., Gutmann E. D., Ferris F. L. Studies with DNA-cellulose chromatography. I. DNA-binding proteins from Escherichia coli. Cold Spring Harb Symp Quant Biol. 1968;33:289–305. doi: 10.1101/sqb.1968.033.01.033. [DOI] [PubMed] [Google Scholar]
  3. Baltimore D. RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature. 1970 Jun 27;226(5252):1209–1211. doi: 10.1038/2261209a0. [DOI] [PubMed] [Google Scholar]
  4. Bosmann H. B. RNA Directed DNA synthesis: Identification in L5178Y mouse leukemic cells and distribution of the polymerase in a synchronized L5178Y cell population. FEBS Lett. 1971 Feb 19;13(2):121–123. doi: 10.1016/0014-5793(71)80214-4. [DOI] [PubMed] [Google Scholar]
  5. Cavalieri L. F., Carroll E. RNA as a template with E. coli DNA polymerase. Biochem Biophys Res Commun. 1970 Nov 25;41(4):1055–1060. doi: 10.1016/0006-291x(70)90192-0. [DOI] [PubMed] [Google Scholar]
  6. Duesberg P. H., Robinson W. S. Nucleic acid and proteins isolated from the Rauscher mouse leukemia virus (MLV). Proc Natl Acad Sci U S A. 1966 Jan;55(1):219–227. doi: 10.1073/pnas.55.1.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Duesberg P., Helm K. V., Canaani E. Properties of a soluble DNA polymerase isolated from Rous sarcoma virus. Proc Natl Acad Sci U S A. 1971 Apr;68(4):747–751. doi: 10.1073/pnas.68.4.747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gallo R. C., Yang S. S., Ting R. C. RNA dependent DNA polymerase of human acute leukaemic cells. Nature. 1970 Dec 5;228(5275):927–929. doi: 10.1038/228927a0. [DOI] [PubMed] [Google Scholar]
  9. Green M., Rokutanda M., Fujinaga K., Ray R. K., Rokutanda H., Gurgo C. Mechanism of carcinogenesis by RNA tumor viruses. I. An RNA-dependent DNA polymerase in murine sarcoma viruses. Proc Natl Acad Sci U S A. 1970 Sep;67(1):385–393. doi: 10.1073/pnas.67.1.385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hatanaka M., Huebner R. J., Gilden R. V. Specificity of the DNA product of the C-type virus RNA-dependent DNA polymerase. Proc Natl Acad Sci U S A. 1971 Jan;68(1):10–12. doi: 10.1073/pnas.68.1.10. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LEE-HUANG S., CAVALIERI L. F. POLYRIBONUCLEOTIDES AS TEMPLATES FOR POLYDEOXYRIBONUCLEOTIDES. Proc Natl Acad Sci U S A. 1963 Dec;50:1116–1122. doi: 10.1073/pnas.50.6.1116. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Penner P. E., Cohen L. H., Loeb L. A. RNA-dependent DNA polymerase: presence in normal human cells. Biochem Biophys Res Commun. 1971 Mar 19;42(6):1228–1234. doi: 10.1016/0006-291x(71)90037-4. [DOI] [PubMed] [Google Scholar]
  13. Scolnick E. M., Aaronson S. A., Todaro G. J., Parks W. P. RNA dependent DNA polymerase activity in mammalian cells. Nature. 1971 Jan 29;229(5283):318–321. doi: 10.1038/229318a0. [DOI] [PubMed] [Google Scholar]
  14. Scolnick E., Rands E., Aaronson S. A., Todaro G. J. RNA-dependent DNA polymerase activity in five RNA viruses: divalent cation requirements. Proc Natl Acad Sci U S A. 1970 Dec;67(4):1789–1796. doi: 10.1073/pnas.67.4.1789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Spiegelman S., Burny A., Das M. R., Keydar J., Schlom J., Travnicek M., Watson K. Characterization of the products of DNA-directed DNA polymerases in oncogenic RNA viruses. Nature. 1970 Aug 8;227(5258):563–567. doi: 10.1038/227563a0. [DOI] [PubMed] [Google Scholar]
  16. Spiegelman S., Burny A., Das M. R., Keydar J., Schlom J., Travnicek M., Watson K. DNA-directed DNA polymerase activity in oncogenic RNA viruses. Nature. 1970 Sep 5;227(5262):1029–1031. doi: 10.1038/2271029a0. [DOI] [PubMed] [Google Scholar]
  17. Spiegelman S., Burny A., Das M. R., Keydar J., Schlom J., Trávnícek M., Watson K. Synthetic DNA-RNA hybrids and RNA-RNA duplexes as templates for the polymerases of the oncogenic RNA viruses. Nature. 1970 Oct 31;228(5270):430–432. doi: 10.1038/228430a0. [DOI] [PubMed] [Google Scholar]
  18. Temin H. M., Mizutani S. RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature. 1970 Jun 27;226(5252):1211–1213. doi: 10.1038/2261211a0. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES