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. 1971 Oct;68(10):2407–2411. doi: 10.1073/pnas.68.10.2407

On the Process of Cellular Division in Escherichia coli: Replication of the Bacterial Chromosome Under Control of Prophage P2*

Gunnar Lindahl 1, Yukinori Hirota 1, François Jacob 1
PMCID: PMC389432  PMID: 4944622

Abstract

The temperature-sensitive mutant CRT46 of Escherichia coli K12 is unable to initiate new rounds of DNA replication at 42°C. Mutants of bacteriophage P2 have been isolated, which, in the prophage state, allow mutant CRT46 to grow at 42°C. The lysogenic bacteria that grow at 42°C are apparently replicating under the control of prophage P2, which substitutes for the bacterial initiation system. The ability of prophage P2 cause this suppression phenomenon depends on the position of the prophage on the bacterial chromosome. Those lysogenic strains that are able to grow at 42°C all carry the prophage close to metE. The P2 mutants that allow CRT46 to grow at 42°C have insertions in the early region of the P2 genome. The suppression requires the cis-acting protein formed by gene A of P2.

Keywords: mutants, met E, cis-acting protein, lysogenic bacteria

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bertani L. E. Abortive induction of bacteriophage P2. Virology. 1968 Sep;36(1):87–103. doi: 10.1016/0042-6822(68)90119-0. [DOI] [PubMed] [Google Scholar]
  2. Brachet P., Eisen H., Rambach A. Mutations of coliphage lambda affecting the expression of replicative functions O and P. Mol Gen Genet. 1970;108(3):266–276. doi: 10.1007/BF00283357. [DOI] [PubMed] [Google Scholar]
  3. Calendar R., Lindahl G. Attachment of prophage P2: gene order at different host chromosomal sites. Virology. 1969 Dec;39(4):867–881. doi: 10.1016/0042-6822(69)90023-3. [DOI] [PubMed] [Google Scholar]
  4. Calendar R., Lindqvist B., Sironi G., Clark A. J. Characterization of REP- mutants and their interaction with P2 phage. Virology. 1970 Jan;40(1):72–83. doi: 10.1016/0042-6822(70)90380-6. [DOI] [PubMed] [Google Scholar]
  5. Hirota Y., Jacob F., Ryter A., Buttin G., Nakai T. On the process of cellular division in Escherichia coli. I. Asymmetrical cell division and production of deoxyribonucleic acid-less bacteria. J Mol Biol. 1968 Jul 14;35(1):175–192. doi: 10.1016/s0022-2836(68)80046-4. [DOI] [PubMed] [Google Scholar]
  6. Hirota Y., Mordoh J., Jacob F. On the process of cellular division in Escherichia coli. 3. Thermosensitive mutants of Escherichia coli altered in the process of DNA initiation. J Mol Biol. 1970 Nov 14;53(3):369–387. doi: 10.1016/0022-2836(70)90072-0. [DOI] [PubMed] [Google Scholar]
  7. Jordan E., Saedler H., Starlinger P. O0 and strong-polar mutations in the gal operon are insertions. Mol Gen Genet. 1968;102(4):353–363. doi: 10.1007/BF00433726. [DOI] [PubMed] [Google Scholar]
  8. KELLY B. Localization of P2 prophage in two strains of Escherichia coli. Virology. 1963 Jan;19:32–39. doi: 10.1016/0042-6822(63)90021-7. [DOI] [PubMed] [Google Scholar]
  9. Kohiyama M., Cousin D., Ryter A., Jacob F. Mutants thermosensibles d'Escherichia coli K 12. I. Isolement et caractérisation rapide. Ann Inst Pasteur (Paris) 1966 Apr;110(4):465–486. [PubMed] [Google Scholar]
  10. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  11. Lindahl G. Bacteriophage P2: replication of the chromosome requires a protein which acts only on the genome that coded for it. Virology. 1970 Oct;42(2):522–533. doi: 10.1016/0042-6822(70)90295-3. [DOI] [PubMed] [Google Scholar]
  12. Lindahl G. Genetic map of bacteriophage P2. Virology. 1969 Dec;39(4):839–860. doi: 10.1016/0042-6822(69)90021-x. [DOI] [PubMed] [Google Scholar]
  13. Lindqvist B. H. Vegetative DNA of temperate coliphage P2. Mol Gen Genet. 1971;110(2):178–196. doi: 10.1007/BF00332647. [DOI] [PubMed] [Google Scholar]
  14. MONOD J., COHEN-BAZIRE G., COHN M. Sur la biosynthèse de la beta-galactosidase (lactase) chez Escherichia coli; la spécificité de l'induction. Biochim Biophys Acta. 1951 Nov;7(4):585–599. doi: 10.1016/0006-3002(51)90072-8. [DOI] [PubMed] [Google Scholar]
  15. Nishimura Y., Caro L., Berg C. M., Hirota Y. Chromosome replication in Escherichia coli. IV. Control of chromosome replication and cell division by an integrated episome. J Mol Biol. 1971 Feb 14;55(3):441–456. doi: 10.1016/0022-2836(71)90328-7. [DOI] [PubMed] [Google Scholar]
  16. Shapiro J. A. Mutations caused by the insertion of genetic material into the galactose operon of Escherichia coli. J Mol Biol. 1969 Feb 28;40(1):93–105. doi: 10.1016/0022-2836(69)90298-8. [DOI] [PubMed] [Google Scholar]

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