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. 1971 Oct;68(10):2604–2607. doi: 10.1073/pnas.68.10.2604

Irreversible Inhibition of Biotin Transport in Yeast by Biotinyl-p-nitrophenyl Ester

Jeffrey M Becker 1, Meir Wilchek 1, Ephraim Katchalski 1
PMCID: PMC389477  PMID: 4944635

Abstract

Biotinyl-p-nitrophenyl ester (BNP), an active-ester derivative of biotin, irreversibly inactivates biotin transport in the yeast Saccharomyces cerevisiae. Transport inactivation is progressive with time and occurs at concentrations of the ester as low as 10-7 M. In the presence of sodium azide, a reagent known to block biotin accumulation in yeast, the derivative is still effective. The specificity of inactivation by the ester is revealed by the following findings: (a) Biotinyl-p-nitroanilide and acetyl-p-nitrophenyl ester do not affect biotin transport; (b) the nitrophenyl ester does not affect the transport of lysine and aspartic acid, or that of L-sorbose; (c) inactivation of biotin transport by the ester is partially prevented when the cells are incubated with it in the presence of relatively high concentrations of biotin.

Keywords: affinity label, biotinyl-p-nitroanilide, acetyl-p-nitrophenyl ester, sorbose transport, amino-acid transport

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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