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. 1971 Nov;68(11):2782–2786. doi: 10.1073/pnas.68.11.2782

Supraoptic Neurosecretory Neurons of the Guinea Pig in Organ Culture. Biosynthesis of Vasopressin and Neurophysin

H Sachs 1,2, R Goodman 1,2, J Osinchak 1,2,*, J McKelvy 1,2
PMCID: PMC389524  PMID: 5288257

Abstract

Fragments of the anterior hypothalamus that contain supraoptic nuclei and short axonal segments from adult guinea pigs have been kept in organ culture for up to 15 days. Electron micrographs displayed intact nuclei, Nissl substance, Golgi bodies, and an ultrastructure characteristic of viable neurosecretory cells; by contrast, the surrounding neurophil showed extensive degeneration. The cultured hypothalamic tissues of the guinea pig that were pulsed with [3H]uridine incorporated label into the RNA of neurosecretory neurons, as determined by radioautography and chemical analysis. Furthermore, and most important, these cells retained a complement of hormones and the ability to incorporate 3H- and 35S-labeled amino acids into vasopressin, neurophysin, and other polypeptides. This incorporation was inhibited by either puromycin or cycloheximide.

Keywords: hypothalamic median eminence, paraventricular nuclei, guinea pig, electron microscopy

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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