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. 1971 Dec;68(12):2972–2976. doi: 10.1073/pnas.68.12.2972

On the Similarity Between the tRNAs of Organelles and Prokaryotes

S A Fairfield 1,2,*, W Edgar Barnett 1,2,†
PMCID: PMC389572  PMID: 4257127

Abstract

Fluorescence studies with organelle transfer RNAs separated from their cytoplasmic counterparts revealed that phenylalanine tRNA from Euglena chloroplasts or Neurospora mitochondria does not contain a fluorescent “base Y.” In contrast, cytoplasmic phenylalanine tRNA from Euglena and cytoplasmic tRNA from Neurospora were found to contain fluorescent bases. The fluorescence-emission spectra of Neurospora cytoplasmic tRNAs and those of the related ascomycete Saccharomyces cerevisiue were observed to be quite different. The results support the generalization that eukaryotic tRNAs contain a fluorescent base, but indicate that their respective organelle tRNAs do not. They also indicate a striking parallelism between organelle and prokaryotic tRNAs.

Keywords: mitochondria, chloroplasts, Euglena gracilis, Neurospora crassa, fluorescent bases

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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