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. 1972 Oct;69(10):3049–3053. doi: 10.1073/pnas.69.10.3049

Hybridization of Variants of Chloramphenicol Acetyltransferase Specified by fi+ and fi- R Factors

William V Shaw *, Linda C Sands *, Naomi Datta †
PMCID: PMC389704  PMID: 4628098

Abstract

An fi- type of R factor for transferable chloramphenicol resistance carries the structural gene for a novel species of chloramphenicol acetyltransferase (EC 2.3.1.99). The enzyme associated with the fi- plasmid is distinct from that described for fi+ R factors. The fi+- and fi--related variants of chloramphenicol acetyltransferase were hybridized in vivo and in vitro. Both techniques yielded only a single symmetrical heteromeric species rather than the three (A3B1, A2B2, and A1B3) hybrids predicted from the tetrameric (identical monomers) structure of chloramphenicol acetyltransferase.

Keywords: episomes, enzyme evolution, antibiotic resistance, plasmids, protein subunits

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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