Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Nov;69(11):3110–3114. doi: 10.1073/pnas.69.11.3110

Formation of Hybrids Between Coliphage λ and Salmonella Phage P22 with a Salmonella typhimurium Hybrid Sensitive to These Phages

P Gemski Jr *, L S Baron *, Nobuto Yamamoto
PMCID: PMC389715  PMID: 4564201

Abstract

An unusual Salmonella typhimurium hybrid with sensitivity to coliphage λ and salmonella phage P22 has been recovered from matings between an Escherichia coli K-12 Hfr donor and an S. typhimurium recipient. The hybrid is an excellent host for achieving genetic recombination between λ and P22. Two broad classes of hybrid phages were isolated. The λ-P22 hybrid class, which has the protein coat of λ, contains at least the c region of P22. The P22-λ hybrid class has the protein coat of P22 and has inherited at least the c marker of λ.

Keywords: intergeneric conjugation, bacterial viruses, recombination

Full text

PDF
3110

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARON L. S., SPILMAN W. M., CAREY W. F. Diploid heterozygous hybrids from matings between Escherichia coli and Salmonella typhosa. J Exp Med. 1960 Aug 1;112:361–372. doi: 10.1084/jem.112.2.361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baron L. S., Penido E., Ryman I. R., Falkow S. Behavior of coliphage lambda in hybrids between Escherichia coli and Salmonella. J Bacteriol. 1970 Apr;102(1):221–233. doi: 10.1128/jb.102.1.221-233.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baron L. S., Ryman I. R., Johnson E. M., Gemski P., Jr Lytic replication of coliphage lambda in Salmonella typhosa hybrids. J Bacteriol. 1972 Jun;110(3):1022–1031. doi: 10.1128/jb.110.3.1022-1031.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Falkow S., Rownd R., Baron L. S. GENETIC HOMOLOGY BETWEEN ESCHERICHIA COLI K-12 AND SALMONELLA. J Bacteriol. 1962 Dec;84(6):1303–1312. doi: 10.1128/jb.84.6.1303-1312.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gemski P., Jr, Stocker B. A. Transduction by bacteriophage P22 in nonsmooth mutants of Salmonella typhimurium. J Bacteriol. 1967 May;93(5):1588–1597. doi: 10.1128/jb.93.5.1588-1597.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KAISER A. D. A genetic study of the temperate coliphage. Virology. 1955 Nov;1(4):424–443. doi: 10.1016/0042-6822(55)90036-2. [DOI] [PubMed] [Google Scholar]
  7. LEVINE M. Mutations in the temperate phage P22 and lysogeny in Salmonella. Virology. 1957 Feb;3(1):22–41. doi: 10.1016/0042-6822(57)90021-1. [DOI] [PubMed] [Google Scholar]
  8. Lieb M. Studies of heat-inducible lambda bacteriophage. I. Order of genetic sites and properties of mutant prophages. J Mol Biol. 1966 Mar;16(1):149–163. doi: 10.1016/s0022-2836(66)80269-3. [DOI] [PubMed] [Google Scholar]
  9. Morse M L, Lederberg E M, Lederberg J. Transductional Heterogenotes in Escherichia Coli. Genetics. 1956 Sep;41(5):758–779. doi: 10.1093/genetics/41.5.758. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sanderson K. E. Current linkage map of Salmonella typhimurium. Bacteriol Rev. 1970 Jun;34(2):176–193. doi: 10.1128/br.34.2.176-193.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Taylor A. L. Current linkage map of Escherichia coli. Bacteriol Rev. 1970 Jun;34(2):155–175. doi: 10.1128/br.34.2.155-175.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wilkinson R. G., Gemski P., Jr, Stocker B. A. Non-smooth mutants of Salmonella typhimurium: differentiation by phage sensitivity and genetic mapping. J Gen Microbiol. 1972 May;70(3):527–554. doi: 10.1099/00221287-70-3-527. [DOI] [PubMed] [Google Scholar]
  13. YAMAMOTO N., ANDERSON T. F. Genomic masking and recombination between serologically unrelated phages P22 and P221. Virology. 1961 Aug;14:430–439. doi: 10.1016/0042-6822(61)90334-8. [DOI] [PubMed] [Google Scholar]
  14. Yamamoto N. Genetic evolution of bacteriophage. I. Hybrids between unrelated bacteriophages P22 and Fels 2. Proc Natl Acad Sci U S A. 1969 Jan;62(1):63–69. doi: 10.1073/pnas.62.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Yamamoto N. The origin of bacteriophage P221. Virology. 1967 Nov;33(3):545–547. doi: 10.1016/0042-6822(67)90132-8. [DOI] [PubMed] [Google Scholar]
  16. ZINDER N. D., LEDERBERG J. Genetic exchange in Salmonella. J Bacteriol. 1952 Nov;64(5):679–699. doi: 10.1128/jb.64.5.679-699.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES