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. 1972 Nov;69(11):3360–3364. doi: 10.1073/pnas.69.11.3360

Degradation of DNA RNA Hybrids by Ribonuclease H and DNA Polymerases of Cellular and Viral Origin

Walter Keller *, Robert Crouch †
PMCID: PMC389771  PMID: 4343966

Abstract

Ribonuclease H from human KB cells, chick embryos, calf thymus, avian myeloblastosis virus, and Rous associated virus specifically degrades the RNA of DNA·RNA hybrids, producing mono- and oligoribonucleotides terminated in 5′-phosphates. The cellular RNase H is an endonuclease, whereas the viral enzyme appears to be an exonuclease. Viral DNA polymerase and RNase H copurify through all separation steps. Therefore, RNase H activity is an intrinsic part of the viral DNA polymerase. DNA·RNA hybrids are also degraded by nucleases associated with cellular DNA polymerases and by exonuclease III. However, these nucleases differ from RNase H in their ability to degrade both strands of DNA·RNA hybrids.

Keywords: RNA tumor viruses, DNA synthesis

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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