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. 1972 Nov;69(11):3440–3442. doi: 10.1073/pnas.69.11.3440

Rapid Axoplasmic Transport of Tyrosine Hydroxylase in Relation to Other Cytoplasmic Constituents

Bevyn Jarrott 1, Laurence B Geffen 1
PMCID: PMC389789  PMID: 4117772

Abstract

The transport of norepinephrine and two key enzymes involved in its synthesis, tyrosine hydroxylase (EC 1.14.3a) and dopamine β-hydroxylase (EC 1.14.2.1), has been studied in relation to other axonal constituents in ligated chicken sciatic nerves. Norepinephrine, tyrosine hydroxylase, and dopamine β-hydroxylase activity all increased proximal to the constriction over a 20-hr period. The rate of transport of norepinephrine, tyrosine hydroxylase, and dopamine β-hydroxylase were calculated as 2.6, 1.9, and 3.4 mm/hr, respectively. The only enzyme marker to show a similar rate of accumulation was acetylcholinesterase (EC 3.1.1.7), a putative marker for endoplasmic reticulum. The rapid axoplasmic transport of tyrosine hydroxylase from the cell bodies to the terminals of sympathetic neurons may be adequate to account for the elevated amounts of this enzyme in chronically active axon terminals, without the necessity to invoke peripheral axonal synthesis of the enzyme to explain such elevated amounts.

Keywords: constricted chicken sciatic nerves, dopamine β-hydroxylase, norepinephrine, acetylcholinesterase

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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