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. 1972 Nov;69(11):3459–3463. doi: 10.1073/pnas.69.11.3459

Induction of Auxotrophic Mutations by Treatment of Chinese Hamster Cells with 5-Bromodeoxyuridine and Black Light

E H Y Chu 1,2,*, N C Sun 1,2,*, C C Chang 1,2,*
PMCID: PMC389793  PMID: 4508333

Abstract

Treatment of an aneuploid Chinese hamster cell line (V79) in tissue culture with 5-bromodeoxyuridine and black light, with or without prior exposure of cells to another mutagen, led to the isolation of auxotrophic mutants, each of which exhibits a specific nutritional requirement for glycine, uridine, purine, or for a combination of glycine, hypoxanthine, and thymidine. 61 mutants that could not use exogenous galactose were also isolated. Thus, this treatment functions as an inductive, but not as a selective, procedure for mutations in mammalian cells.

Keywords: mutagenesis, nutritional requirements, mammalian cell culture

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Selected References

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