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. 1972 Dec;69(12):3810–3814. doi: 10.1073/pnas.69.12.3810

Replication of Mitochondrial DNA in Mouse L Cells and Their Thymidine Kinase- Derivatives: Displacement Replication on a Covalently-Closed Circular Template

Donald L Robberson 1, David A Clayton 1
PMCID: PMC389878  PMID: 4509344

Abstract

Thymidine kinase- lines of mouse L cells incorporate thymidine exclusively into mitochondrial DNA. This fact permits assessment of labeled mitochondrial DNA components in ethidium bromide-cesium chloride gradients. Contaminating nuclear DNA is unlabeled and need not be removed. Elimination of the DNase treatment of purified mitochondria reveals that the replicative forms that exhibit displacement replication up to full genome size are covalently-closed circular molecules. Denaturation followed by brief renaturation of these larger replicating molecules produces closed-circular DNA with a deficiency of Watson-Crick turns, appearing as single-strand loops. This result suggests that displacement replication proceeds with nicking and rapid closure of the covalently-closed circular template.

Keywords: electron microscopy, mouse DNA, density gradient centrifugation

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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