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. 1983 Dec;80(23):7284–7288. doi: 10.1073/pnas.80.23.7284

Site-specific recombination of yeast 2-micron DNA in vitro.

D Vetter, B J Andrews, L Roberts-Beatty, P D Sadowski
PMCID: PMC390039  PMID: 6316354

Abstract

Most strains of the yeast Saccharomyces cerevisiae harbor several copies of a 2-micron plasmid circle DNA termed "2 micron." This circular plasmid contains two 599-base-pair precise inverted repeats across which a site-specific inversion event occurs in vivo. This inversion is promoted by a plasmid-encoded function called "FLP." We have cloned the FLP gene of 2-micron DNA under control of a strong yeast promoter and transformed yeast cells with a plasmid containing the cloned FLP gene. Cell-free extracts from such a transformant promote highly efficient inversion of 2-micron DNA in vitro. The reaction requires a cation and works efficiently on supercoiled, relaxed circular, or linear DNA. The FLP activity bears certain similarities to the cre protein, a site-specific recombinase encoded by bacteriophage P1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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