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. 1985 Jul;82(14):4668–4672. doi: 10.1073/pnas.82.14.4668

Origin and turnover of mitochondrial glutathione.

O W Griffith, A Meister
PMCID: PMC390447  PMID: 3860816

Abstract

Mitochondrial glutathione in liver does not arise by intramitochondrial synthesis, but rather from the cytoplasm, by a process characterized by slow net transport and more rapid exchange transport.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boettcher B., Meister A. Synthesis of L-2-oxothiazolidine-4-carboxylic acid. Anal Biochem. 1984 May 1;138(2):449–450. doi: 10.1016/0003-2697(84)90837-6. [DOI] [PubMed] [Google Scholar]
  2. Greenawalt J. W. The isolation of outer and inner mitochondrial membranes. Methods Enzymol. 1974;31:310–323. doi: 10.1016/0076-6879(74)31033-6. [DOI] [PubMed] [Google Scholar]
  3. Griffith O. W. Depletion of glutathione by inhibition of biosynthesis. Methods Enzymol. 1981;77:59–63. doi: 10.1016/s0076-6879(81)77011-3. [DOI] [PubMed] [Google Scholar]
  4. Griffith O. W. Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. Anal Biochem. 1980 Jul 15;106(1):207–212. doi: 10.1016/0003-2697(80)90139-6. [DOI] [PubMed] [Google Scholar]
  5. Griffith O. W., Larsson A., Meister A. Inhibition of gamma-glutamylcysteine synthetase by cystamine: an approach to a therapy of 5-oxoprolinuria (pyroglutamic aciduria). Biochem Biophys Res Commun. 1977 Dec 7;79(3):919–925. doi: 10.1016/0006-291x(77)91198-6. [DOI] [PubMed] [Google Scholar]
  6. Griffith O. W., Meister A. Glutathione: interorgan translocation, turnover, and metabolism. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5606–5610. doi: 10.1073/pnas.76.11.5606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Griffith O. W., Meister A. Potent and specific inhibition of glutathione synthesis by buthionine sulfoximine (S-n-butyl homocysteine sulfoximine). J Biol Chem. 1979 Aug 25;254(16):7558–7560. [PubMed] [Google Scholar]
  8. Hartree E. F. Determination of protein: a modification of the Lowry method that gives a linear photometric response. Anal Biochem. 1972 Aug;48(2):422–427. doi: 10.1016/0003-2697(72)90094-2. [DOI] [PubMed] [Google Scholar]
  9. Higashi T., Tateishi N., Naruse A., Sakamoto Y. A novel physiological role of liver glutathione as a reservoir of L-cysteine. J Biochem. 1977 Jul;82(1):117–124. doi: 10.1093/oxfordjournals.jbchem.a131659. [DOI] [PubMed] [Google Scholar]
  10. Högberg J., Kristoferson A. Glutathione turnover in isolated hepatocytes. Acta Pharmacol Toxicol (Copenh) 1978 Apr;42(4):271–274. doi: 10.1111/j.1600-0773.1978.tb02200.x. [DOI] [PubMed] [Google Scholar]
  11. Jocelyn P. C., Kamminga A. The non-protein thiol of rat liver mitochondria. Biochim Biophys Acta. 1974 Apr 22;343(2):356–362. doi: 10.1016/0304-4165(74)90099-3. [DOI] [PubMed] [Google Scholar]
  12. Jocelyn P. C. Some properties of mitochondrial glutathione. Biochim Biophys Acta. 1975 Sep 8;396(3):427–436. doi: 10.1016/0005-2728(75)90148-6. [DOI] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Lauterburg B. H., Mitchell J. R. Regulation of hepatic glutathione turnover in rats in vivo and evidence for kinetic homogeneity of the hepatic glutathione pool. J Clin Invest. 1981 May;67(5):1415–1424. doi: 10.1172/JCI110170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Meredith M. J., Reed D. J. Status of the mitochondrial pool of glutathione in the isolated hepatocyte. J Biol Chem. 1982 Apr 10;257(7):3747–3753. [PubMed] [Google Scholar]
  16. Newton G. L., Dorian R., Fahey R. C. Analysis of biological thiols: derivatization with monobromobimane and separation by reverse-phase high-performance liquid chromatography. Anal Biochem. 1981 Jul 1;114(2):383–387. doi: 10.1016/0003-2697(81)90498-x. [DOI] [PubMed] [Google Scholar]
  17. Oppenheimer L., Wellner V. P., Griffith O. W., Meister A. Glutathione synthetase. Purification from rat kidney and mapping of the substrate binding sites. J Biol Chem. 1979 Jun 25;254(12):5184–5190. [PubMed] [Google Scholar]
  18. Romero F. J., Sies H. Subcellular glutathione contents in isolated hepatocytes treated with L-buthionine sulfoximine. Biochem Biophys Res Commun. 1984 Sep 28;123(3):1116–1121. doi: 10.1016/s0006-291x(84)80248-x. [DOI] [PubMed] [Google Scholar]
  19. Sekura R., Meister A. Glutathione turnover in the kidney; considerations relating to the gamma-glutamyl cycle and the transport of amino acids. Proc Natl Acad Sci U S A. 1974 Aug;71(8):2969–2972. doi: 10.1073/pnas.71.8.2969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Taniguchi N., Meister A. gamma-Glutamyl cyclotransferase from rat kidney. Sulfhydryl groups and isolation of a stable form of the enzyme. J Biol Chem. 1978 Mar 25;253(6):1799–1806. [PubMed] [Google Scholar]
  21. Tate S. S., Meister A. Interaction of gamma-glutamyl transpeptidase with amino acids, dipeptides, and derivatives and analogs of glutathione. J Biol Chem. 1974 Dec 10;249(23):7593–7602. [PubMed] [Google Scholar]
  22. Tietze F. Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem. 1969 Mar;27(3):502–522. doi: 10.1016/0003-2697(69)90064-5. [DOI] [PubMed] [Google Scholar]
  23. Van der Werf P., Orlowski M., Meister A. Enzymatic conversion of 5-oxo-L-proline (L-pyrrolidone carboxylate) to L-glutamate coupled with cleavage of adenosine triphosphate to adenosine diphosphate, a reaction in the -glutamyl cycle. Proc Natl Acad Sci U S A. 1971 Dec;68(12):2982–2985. doi: 10.1073/pnas.68.12.2982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wahlländer A., Soboll S., Sies H., Linke I., Müller M. Hepatic mitochondrial and cytosolic glutathione content and the subcellular distribution of GSH-S-transferases. FEBS Lett. 1979 Jan 1;97(1):138–140. doi: 10.1016/0014-5793(79)80069-1. [DOI] [PubMed] [Google Scholar]
  25. Williamson J. M., Boettcher B., Meister A. Intracellular cysteine delivery system that protects against toxicity by promoting glutathione synthesis. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6246–6249. doi: 10.1073/pnas.79.20.6246. [DOI] [PMC free article] [PubMed] [Google Scholar]

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